Chronic β-adrenergic stimulation induces myocardial proinflammatory cytokine expression
Chronic β-adrenergic stimulation induces myocardial proinflammatory cytokine expression
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DOI：
10.1161/01.cir.101.20.2338
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发表时间：
2000-05-23
期刊：
影响因子：
37.8
通讯作者：
Chandrasekar, B
中科院分区：
文献类型：
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作者：
Murray, DR;Prabhu, SD;Chandrasekar, B
背景-交感神经系统和促炎性细胞因子被认为在充血性心力衰竭的病理生理学中起关键作用。为了评估这些神经激素系统之间的可能关系，我们研究了慢性β-肾上腺素能刺激对心肌和全身肿瘤坏死因子-α、白介素1-β和白介素6分泌的影响。方法与结果：雄性大鼠给予L-异丙肾上腺素(2.4 mg。Kg(-1)。D(-1，n=8)或生理盐水(n=7)。Northern和Western印迹分析心肌细胞因子的表达，免疫组织化学定位心肌细胞因子的表达。采用酶联免疫吸附试验检测循环中细胞因子水平。在对照组动物的心肌中，既没有检测到肿瘤坏死因子α，也没有检测到IL-1β，而IL-6存在于非常低的水平。异丙肾上腺素引起显著(P<0.05)。
Background-The sympathetic nervous system and proinflammatory cytokines are believed to play key roles in the pathophysiology of congestive heart failure. To evaluate a possible relationship between these neurohormonal systems, we studied the effects of chronic beta-adrenergic stimulation on the myocardial and systemic elaboration of tumor necrosis factor (TNF)-alpha, interleukin (LL)-1 beta, and IL-6.Methods and Results-Male rats received either L-isoproterenol (2.4 mg . kg(-1) . d(-1), n=8) or saline (n=7) via miniosmotic pumps for 7 days. Myocardial cytokine expression was analyzed by both Northern and Western blotting and localized in the tissue using immunohistochemistry. ELISA was performed to measure circulating levels of cytokines. In myocardium from control animals, neither TNF-alpha nor IL-1 beta were detected, whereas IL-6 was present at very low levels. Isoproterenol led to a significant (P