Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model
Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model
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DOI：
10.1016/j.exer.2015.01.020
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发表时间：
2015-03-01
影响因子：
3.4
通讯作者：
De Miguel, Maria P.
中科院分区：
文献类型：
--
作者：
Alio del Barrio, Jorge L.;Chiesa, Massimo;De Miguel, Maria P.
目的：评价脱细胞人角膜基质与人脂肪来源的成体干细胞(h-ADASC)复合移植到兔角膜体内的生物相容性。方法：对90g厚的人角膜基质进行脱细胞，检测其细胞毒性。通过在移植物内注射2×10(5)标记的h-ADASC，然后进行5天的细胞培养，实现了再细胞化。移植物在体内植入50%深度的基质袋内。结果：脱细胞基质片细胞外基质完整，脱细胞率为92.8%，体外培养的h-ADASC具有良好的细胞再生能力。在完全的随访中观察到了完全和稳定的移植物透明，没有任何临床上的排斥迹象。死后分析表明移植的人干细胞在移植物内存活并分化为功能性角膜细胞。结论：我们报道了一种新的板层角膜移植模型，该模型只需要简单安全的吸脂和供体同种异体角膜，在兔模型中提供具有良好的生物相容性和与宿主组织整合的光学透明的自体基质移植物。(C)2015爱思唯尔有限公司。保留所有权利。
Purpose: To evaluate the in vivo biocompatibility of grafts composed of sheets of decellularized human corneal stroma with or without the recellularization of human adipose derived adult stem cells (h-ADASC) into the rabbit cornea.Methods: Sheets of human corneal stroma of 90 gm thickness were decellularized, and their lack of cytotoxicity was assayed. The recellularization was achieved by the injection of 2 x 10(5) labeled h-ADASC in the graft followed by five days of cell culture. The grafts were implanted in vivo into a stromal pocket at 50% depth. After a triple-masked three-month follow-up, the animals were euthanized and the bio-integration of the graft, the viability of the stem cells and the expression of keratocan (human keratocyte-specific protein) were assessed.Results: The decellularized stromal sheets showed an intact extracellular matrix with a decellularization rate of 92.8% and an excellent recellularization capacity in vitro with h-ADASC. A complete and stable graft transparency was observed during the full follow-up, with absence of any clinical sign of rejection. The postmortem analysis demonstrated the survival of the transplanted human stem cells inside the graft and their differentiation into functional keratocytes, as assessed by the expression of human keratocan.Conclusions: We report a new model of lamellar keratoplasty that requires only a simple and safe procedure of liposuction and a donor allogeneic cornea to provide an optically transparent autologous stromal graft with excellent biocompatibility and integration into the host tissue in a rabbit model. (C) 2015 Elsevier Ltd. All rights reserved.