Comparative analysis of the locus control region of the rabbit beta-like gene cluster: HS3 increases transient expression of an embryonic epsilon-globin gene.
Comparative analysis of the locus control region of the rabbit beta-like gene cluster: HS3 increases transient expression of an embryonic epsilon-globin gene.
复制标题
兔β样基因簇基因座控制区的比较分析：HS3增加胚胎ε-珠蛋白基因的瞬时表达。
DOI：
10.1093/nar/21.5.1265
复制
发表时间：
1993
影响因子：
14.9
通讯作者：
Miller,W
中科院分区：
文献类型：
--
作者：
Hardison,R;Xu,J;Jackson,J;Mansberger,J;Selifonova,O;Grotch,B;Biesecker,J;Petrykowska,H;Miller,W
分离了人β-like globlin基因簇的基因座控制区（LCR）的兔同源物，并对含有DNase I超敏位点（HS）的长片段进行了测序。HS 4、HS 3、HS 2和HS 1的顺序和间隔在兔和人之间是保守的。将这些序列与人类、山羊和小鼠的同源物进行比对，发现物种之间的DNA片段非常长，在先前鉴定的功能核心的两侧有超过1000个碱基对，这表明在核心之外发现了一些重要的功能。兔HS 2和HS 3的活性通过将其各自连接到通过将荧光素酶编码区插入兔ε-globln基因而构建的新报告基因来测试。与先前显示人或小鼠HS 3对瞬时表达无影响的报道相反，兔HS 2和HS 3 DNA片段分别增加了K562红白血病细胞中ε-荧光素酶杂合基因的瞬时表达和稳定整合的构建体的表达。
The rabbit homolog to the locus control region (LCR) of the human β-like globln gene cluster was isolated, and long segments containing the DNase I hypersensitive sites (HS) were sequenced. The order and spacing of HS4, HS3, HS2 and HS1 are conserved between rabbit and human. Alignment of these sequences with their homologs from human, goat, and mouse shows that very long segments of DNA match between species, for over a thousand base pairs on either side of the previously identified functional cores, Indicating that some important functions are found outside the cores. The activity of rabbit HS2 and HS3 was tested by attaching each to a novel reporter gene constructed by inserting the luciferase coding region into the rabbit ε-globln gene. In contrast to previous reports showing no effect of human or mouse HS3 on transient expression, both the rabbit HS2 and HS3 DNA fragments separately increased transient expression from the ε-luciferase hybrid gene and expression from stably Integrated constructs in K562 erythroleukemia cells.