Sensitivity to a CD4 mimic of a consensus clone of monkey-adapted CCR5-tropic SHIV-MK38C
Sensitivity to a CD4 mimic of a consensus clone of monkey-adapted CCR5-tropic SHIV-MK38C
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DOI：
10.1016/j.virol.2022.12.004
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发表时间：
2022-12-28
期刊：
影响因子：
3.7
通讯作者：
Miura，Tomoyuki
中科院分区：
文献类型：
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作者：
Matsuura，Kanako;Yamaura，Mizuki;Miura，Tomoyuki
通过使嗜CCR 5的1B级SHIV-MK1适应恒河猴，产生了具有中和抗性和高复制能力的2级SHIV-MK38株。在这项研究中，我们产生了SHIV-MK38 C，这是SHIV-MK38的猴感染性共有分子克隆。使用假型病毒的分析显示，MK38 C是1C级，因为它缺乏N169 D突变，这是中和抗性的最重要突变。携带N169D突变的MK38C成为2级。然而，带有N169D的SHIV-MK38 C的复制能力很低;在猴子中检测到它之前已经过了17周。第1C层MK38C对CD4模拟物敏感。因此，SHIV-MK 38C可用于体内评价CD4模拟。
By acclimatizing CCR5-tropic tier 1B SHIV-MK1 to rhesus monkeys, a tier 2 SHIV-MK38 strain with neutralization resistance and high replication ability was generated. In this study, we generated SHIV-MK38C, a monkey-infectious consensus molecular clone of SHIV-MK38. Analysis using pseudotype viruses showed that MK38C was tier 1C because it lacked the N169D mutation, which is the most important mutation for neutralization resistance. MK38C harboring the N169D mutation became tier 2. However, the replication ability of SHIV-MK38C with N169D was low; more than 17 weeks elapsed before its detection in monkeys. Tier 1C MK38C was sensitive to a CD4 mimic. Therefore, SHIV-MK38C could be used to evaluate CD4 mimicsin vivo.