Anatomy of the parp gene promoter of Trypanosoma brucei.
Anatomy of the parp gene promoter of Trypanosoma brucei.
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布氏锥虫 parp 基因启动子的解剖。
DOI：
10.1002/j.1460-2075.1991.tb04902.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Clayton,C
中科院分区：
文献类型：
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作者：
Sherman,DR;Janz,L;Hug,M;Clayton,C
在采采蝇体内生长时，布氏锥虫表达一种主要的表面糖蛋白，即前环酸性重复蛋白（PARP）。parp基因由α鹅膏蕈碱抗性RNA聚合酶转录。我们已经确定了parp启动子活性的序列要求。对瞬时转染锥虫中输入DNA产生的RNA的研究表明，RNA通过反式剪接和聚腺苷酸化正确加工。缺失分析表明，330 bp是足够的完整的启动子和剪接活性和启动子结构是复杂的，涉及至少三个元件，其相互间距是重要的。诱变作用确定了两个对启动子活性至关重要的序列;它们与已知的原核或真核启动子元件都不相似。
While growing in the tsetse fly, Trypanosoma brucei expresses a major surface glycoprotein, the procyclic acidic repetitive protein (PARP). The parp genes are transcribed by an alpha‐amanitin‐resistant RNA polymerase. We have determined the sequence requirements for parp promoter activity. Studies of RNA produced from input DNA in transiently transfected trypanosomes indicate that the RNA is correctly processed by trans‐splicing and polyadenylation. Deletion analyses show that 330 bp are sufficient for full promoter and splicing activity and that the promoter structure is complex, involving at least three elements whose mutual spacing is important. Mutagenesis pin‐pointed two sequences vital for promoter activity; neither bears any resemblance to known prokaryotic or eukaryotic promoter elements.