Expression of the ecto-ATPase NTPDase2 in the germinal zones of the developing and adult rat brain
Expression of the ecto-ATPase NTPDase2 in the germinal zones of the developing and adult rat brain
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DOI：
10.1046/j.1460-9568.2003.02567.x
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发表时间：
2003-04-01
影响因子：
3.4
通讯作者：
Zimmermann, H
中科院分区：
文献类型：
--
作者：
Braun, N;Sévigny, J;Zimmermann, H
在成人神经系统中，侧脑室的脑室下区的多能干细胞产生神经元前体（A型细胞），其经由吻侧迁移流迁移到嗅球，在嗅球中它们分化成神经元。迁移的神经母细胞被星形胶质细胞（B型细胞）鞘包围。使用免疫染色，原位杂交和酶组织化学，我们表明，外ATP酶核苷三磷酸二磷酸水解酶2（NTPDase2）表达在脑室下区和吻侧迁移流的成年大鼠大脑。这种酶将细胞外核苷三磷酸水解为相应的核苷二磷酸，并被认为直接调节ATP受体介导的细胞通讯。星形胶质细胞中间丝蛋白GFAP和神经胶质谷氨酸转运蛋白GLAST的双标记鉴定NTPD酶2阳性细胞为B型细胞。在发育过程中，在E18首次检测到酶蛋白，早在星形胶质细胞标志物GFAP表达之前。它逐渐沿着脑的脑室和脑室下区表达，随后在P21时完全回缩至成人表达模式。NTPD酶2在齿状回的外分子层和小脑白色物质内瞬时表达，并与成人脑的选择性微血管、第三脑室的伸长细胞和软膜下星形胶质细胞相关。我们的研究结果表明，NTPDase2可以作为一种新的标记物，指定在体内和体外研究的神经发育的细胞亚群，并提高ATP介导的信号通路在神经发育和分化中发挥作用的可能性。
In the adult nervous system, multipotential stem cells of the subventricular zone of the lateral ventricles generate neuron precursors (type-A cells) that migrate via the rostral migratory stream to the olfactory bulb where they differentiate into neurons. The migrating neuroblasts are surrounded by a sheath of astrocytes (type-B cells). Using immunostaining, in situ hybridization and enzyme histochemistry, we demonstrate that the ecto-ATPase nucleoside triphosphate diphosphohydrolase 2 (NTPDase2) is expressed in the subventricular zone and the rostral migratory stream of the adult rat brain. This enzyme hydrolyses extracellular nucleoside triphosphates to the respective nucleoside diphosphates and is thought to directly modulate ATP receptor-mediated cell communication. Double labelling for the astrocyte intermediate filament protein GFAP and the glial glutamate transporter GLAST identifies the NTPDase2-positive cells as type-B cells. During development the enzyme protein is first detected at E18, long before expression of the astrocyte marker GFAP. It gradually becomes expressed along the ventricular and subventricular zone of the brain, followed by complete retraction to the adult expression pattern at P21. NTPDase2 is transiently expressed in the outer molecular layer of the dentate gyrus and within the cerebellar white matter and is associated with select microvessels, tanycytes of the third ventricle, and subpial astrocytes of the adult brain. Our results suggest that NTPDase2 can serve as a novel marker for specifying subsets of cells during in vivo and in vitro studies of neural development and raise the possibility that ATP-mediated signalling pathways play a role in neural development and differentiation.