Comparison of nitrergic signaling in circular and longitudinal smooth muscle of murine ileum
Comparison of nitrergic signaling in circular and longitudinal smooth muscle of murine ileum
复制标题
小鼠回肠环形和纵向平滑肌中氮能信号的比较
DOI：
10.1111/nmo.13175
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发表时间：
2018
影响因子：
3.5
通讯作者：
Friebe A
中科院分区：
文献类型：
--
作者：
Voussen B;Beck K;Mauro N;Keppler J;Friebe A
胃肠(GI)运动起源于环状(CM)和纵向(LM)平滑肌的协调运动。这两个肌肉层如何对氮能输入做出单独的反应，以及它们是如何整合氮能信号的，目前还不完全清楚。方法我们用免疫组织化学方法揭示了回肠中非敏感鸟苷环化酶(NO-GC)的表达。为了进行功能分析，我们测量了整体缺乏NO-GC的小鼠(GCKO)和SMC-GCKO(SMC-GCKO)的回肠肌层的肌张力和自发性收缩。关键结果是，与胃肠道的其他部分不同，回肠cKit阳性细胞中不表达NO-GC。NO-GC在血小板衍生生长因子受体α阳性细胞以及尚未鉴定的肌间神经丛和浆膜细胞中表达强烈。CM和LM均表现出自发收缩活动，其自发收缩的频率和持续时间相同。无NO-GC时，CM的自发性收缩幅度增加。在对照(CTRL)动物回肠中，NO-GC的抑制增加，而NO-GC的刺激降低了组织张力。相反，在ctrl和基因敲除菌株之间，Lm的收缩活动没有区别。在这里，NO导致抑制ctrl回肠的自发收缩，而GCKO组织不受影响。NO抑制SMC-GCKO回肠自发性收缩，提示有其他细胞参与(S)。结论SMC中NO-GC参与了回肠CM自发性收缩的张力和幅度的调节。在Lm，NO通过NO-GC抑制非SMC类型的自发性收缩。
BackgroundGastrointestinal (GI) motility originates from coordinated movements of circular (CM) and longitudinal (LM) smooth muscle. How the two muscle layers react individually to nitrergic input and how they integrate nitrergic signaling is not thoroughly understood.MethodsWe used immunohistochemistry to unveil expression of NO‐sensitive guanylyl cyclase (NO‐GC) in the ileum. For functional analyses, we measured tone of ileal CM and spontaneous contractions in both ileal muscle layers from mice lacking NO‐GC globally (GCKO) and specifically in smooth muscle cells (SMC‐GCKO).Key ResultsIn contrast to other parts of the GI tract, NO‐GC was not expressed in ckit‐positive cells in ileum. NO‐GC expression was intense in platelet‐derived growth factor receptor α‐positive cells and in yet unidentified cells of myenteric plexus and serosa. Both CM and LM developed spontaneous contractile activity; frequency and duration of their spontaneous contractions were identical. The amplitude of spontaneous contractions in CM was increased in the absence of NO‐GC. In ileum from control (ctrl) animals, inhibition of NO‐GC increased whereas NO‐GC stimulation decreased tissue tone. In contrast, contractile activity in LM was not different between ctrl and knockout strains. Here, NO led to suppression of spontaneous contractions of ctrl ileum whereas GCKO tissue was unaffected. To our surprise, NO suppressed spontaneous contractions in SMC‐GCKO ileum indicating participation of other cell type(s).Conclusions and InferencesNO‐GC in SMC is involved in the regulation of tone and amplitude of spontaneous contractions in ileal CM. In LM, NO induces suppression of spontaneous contractions via NO‐GC in a non‐SMC type.
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影响因子：
29.4
作者：
Huh WJ;Khurana SS;Geahlen JH;Kohli K;Waller RA;Mills JC
通讯作者：
Mills JC
豚鼠回肠形态特征肌间神经元粘膜机械刺激引起的突触反应
影响因子：
5.3
作者：
J. Bornstein;J. Furness;T. Smith;D. Trussell
通讯作者：
D. Trussell
介导一氧化氮诱导的人空肠纵向平滑肌抑制的机制。
DOI：
10.1067/msy.2001.116414
发表时间：
2001
期刊：
Surgery.
影响因子：
--
作者：
Zyromski,NJ;Duenes,JA;Kendrick,ML;Balsiger,BM;Farrugia,G;Sarr,MG
通讯作者：
Sarr,MG
粘膜扩张和机械刺激所激发的反射通路会聚到豚鼠小肠的相同肌间神经元上
影响因子：
5.3
作者：
T. Smith;J. Bornstein;J. Furness
通讯作者：
J. Furness
一氧化氮、血管活性肠多肽和 ATP 在人空肠抑制性神经传递中的作用。
DOI：
10.1006/jsre.1999.5590
发表时间：
1999
期刊：
The Journal of surgical research.
影响因子：
--
作者：
Murr,MM;Balsiger,BM;Farrugia,G;Sarr,MG
通讯作者：
Sarr,MG