Electroacupuncture Pretreatment Alleviates Cerebral Ischemia-Reperfusion Injury by Increasing GSK-3β Phosphorylation Level via Adenosine A1 Receptor
Electroacupuncture Pretreatment Alleviates Cerebral Ischemia-Reperfusion Injury by Increasing GSK-3β Phosphorylation Level via Adenosine A1 Receptor
复制标题
电针预处理通过腺苷 A1 受体增加 GSK-3 β 磷酸化水平减轻脑缺血再灌注损伤
DOI：
10.1155/2020/6848450
复制
发表时间：
2020-02-21
影响因子：
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通讯作者：
Wang, Junlu
中科院分区：
文献类型：
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作者：
Geng, Wujun;Cai, Libin;Wang, Junlu
目标。观察小鼠海马腺苷A1受体对GSK-3β磷酸化水平的影响，探讨电针预处理通过激活Alpha1受体介导脑缺血再灌注损伤的可能机制。方法。建立大鼠大脑中动脉闭塞(MCAO)模型，分为电针预处理组(EA组)、MCAO组和假手术组(Sham组)。观察各组小鼠的神经行为表现、脑梗塞体积及其相关蛋白的变化。然后用腺苷Alpha1受体拮抗剂和激动剂腹腔注射，观察A1受体对GSK-3β磷酸化水平、神经行为改变和脑梗塞体积的影响。结果。(1)与MCAO组(再灌注后24小时)比较，电针组脑梗塞面积明显缩小，Garcia神经功能评分和GSK-3β磷酸化水平升高。(2)与电针组比较，A1受体拮抗剂8-环戊基-1，3-二丙基黄嘌呤(DPCPX)组脑梗塞面积明显扩大，GSK-3β磷酸化水平和Garcia神经功能评分降低。(3)A1受体激动剂2-氯-N6-环戊基腺苷(CCPA)组与MCAO组比较，脑梗塞面积明显缩小，GSK-3β磷酸化水平和Garcia神经功能评分明显升高。电针组与CCPA组比较差异无统计学意义。结论。电针预处理可通过激活A1受体，提高GSK-3β的磷酸化水平，对缺血再灌注损伤的神经元起到保护作用。
Objective. To observe the effect of adenosine A1 receptor in the hippocampus of mice on GSK-3 beta phosphorylation level and elucidate the underlying mechanisms of electroacupuncture pretreatment by activating Alpha 1 receptor mediating cerebral ischemia-reperfusion injury. Method. The model of middle cerebral artery occlusion (MCAO) was established and grouped into electroacupuncture pretreatment group (EA group), MCAO group, and sham-operated group (Sham group). The neurobehavioral manifestation, the volume of cerebral infarction, and its related protein changes in mice in each group were observed. Then, adenosine Alpha 1 receptor antagonist and agonist were injected intraperitoneally to observe the effects of A1 receptor on the phosphorylation level of GSK-3 beta, neurobehavioral changes, and infarction volume. Results. (1) Compared with the MCAO group (24 hours after reperfusion), the infarct size in the EA group decreased significantly, and the Garcia neurological score and phosphorylation level of GSK-3 beta are increased. (2) Compared with the EA group, the infarct size in the A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) group increased significantly, and the Garcia neurological score and phosphorylation level of GSK-3 beta are decreased. (3) Compared with the MCAO group, the infarct size in the A1 receptor agonist 2-Chloro-N6-cyclopentyladenosine (CCPA) group decreased significantly, and the Garcia neurological score and phosphorylation level of GSK-3 beta are increased. There was no significant difference between the EA group and CCPA group. Conclusions. Electroacupuncture pretreatment can increase GSK-3 beta phosphorylation level via activating A1 receptor, to protect neurons in ischemia-reperfusion injury.