Differential effects of retinoic acid upon early and late events in adipose conversion of 3T3 preadipocytes.
Differential effects of retinoic acid upon early and late events in adipose conversion of 3T3 preadipocytes.
复制标题
视黄酸对 3T3 前脂肪细胞脂肪转化的早期和晚期事件的不同影响。
DOI：
10.1016/0014-4827(88)90022-5
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发表时间：
1988
影响因子：
3.7
通讯作者：
F. Lasnier
中科院分区：
文献类型：
--
作者：
J. Pairault;A. Quignard‐Boulangé;I. Dugail;F. Lasnier
当汇合的3 T3-F442 A细胞培养物（第0天）在含有异丁基甲基黄嘌呤和地塞米松的补充胎牛血清的培养基中生长3天时（诱导阶段），然后转移至无血清琼脂糖确定培养基（表达期），它们自发地表现出脂蛋白脂酶活性（LPL）的急剧上升;在峰值（第7天），LPL活性比对照培养物中高约25倍，并且被胰岛素进一步增强。虽然这种LPL活性的表达是自发的，甘油磷酸脱氢酶（G3 PDH）活性的出现完全依赖于胰岛素以及分化表型的表达。在定向细胞中，胰岛素引起与有限细胞增殖相关的持续DNA合成。在表达阶段添加视黄酸抑制胰岛素依赖性终末分化（即，G3 PDH活性的出现和分化表型的获得）。此外，视黄酸可抵消胰岛素对LPL活性的刺激作用，但不影响有丝分裂过程，也不影响LPL活性的自发出现。当在诱导阶段加入时，它阻止了脂肪形成分化的整个过程。因此，视黄酸的使用可以指示在致力于脂肪形成分化之后胰岛素的促有丝分裂和脂肪形成作用的独立控制。
When confluent 3T3-F442A cell cultures (Day 0) were grown for 3 days in fetal calf serum-supplemented medium containing isobutyl methyl xanthine and dexamethasone (induction phase) and then shifted to serum-free hormone-defined medium (expression phase), they spontaneously exhibited a sharp rise in lipoprotein lipase activity (LPL); at the peak (Day 7) the LPL activity was about 25 times higher than in control cultures and was further enhanced by insulin. Although this expression of LPL activity was spontaneous, the emergence of glycerophosphate dehydrogenase (G3PDH) activity was completely dependent upon insulin as well as upon the expression of the differentiated phenotype. In committed cells, insulin elicited sustained DNA synthesis associated with limited cell proliferation. The addition of retinoic acid during the phase of expression inhibited insulin-dependent terminal differentiation (i.e., the emergence of G3PDH activity and acquisition of the differentiated phenotype). In addition, retinoic acid counteracted the stimulating effect of insulin upon LPL activity, but affected neither the mitotic process nor the spontaneous emergence of LPL activity. When added during the phase of induction, it prevented the overall process of adipogenic differentiation. Thus, the use of retinoic acid can indicate independent control of the mitogenic and lipogenic effects of insulin following commitment to adipogenic differentiation.