QUIESCENT LYMPHOCYTES-T AS AN INDUCIBLE VIRUS RESERVOIR IN HIV-1 INFECTION
QUIESCENT LYMPHOCYTES-T AS AN INDUCIBLE VIRUS RESERVOIR IN HIV-1 INFECTION
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DOI：
10.1126/science.1925601
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发表时间：
1991-10-18
期刊：
影响因子：
56.9
通讯作者：
STEVENSON, M
中科院分区：
文献类型：
--
作者：
BUKRINSKY, MI;STANWICK, TL;STEVENSON, M
为了更好地理解1型人类免疫缺陷病毒（HIV - 1）持续存在和潜伏的基础，对感染个体外周T淋巴细胞储存库中病毒DNA存在的形式进行了研究。在无症状个体中，HIV - 1主要以全长、未整合的互补DNA形式存在。这些染色体外DNA形式在体外T细胞激活时保留了整合能力。在获得性免疫缺陷综合征（艾滋病）患者中，整合形式相对于染色体外DNA形式有所增加。通过对去除人类淋巴细胞抗原 - Dr受体阳性细胞的患者淋巴细胞亚群的DNA分析，静止T细胞被确定为染色体外HIV - 1 DNA的来源。因此，静止T淋巴细胞可能是感染个体中主要的且可诱导的HIV - 1储存库。
To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-11 reservoir in infected individuals.