PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY
PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY
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DOI：
10.1042/bj0890114
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发表时间：
1963-01-01
影响因子：
4.1
通讯作者：
HUNTER, WM
中科院分区：
文献类型：
--
作者：
GREENWOOD, FC;HUNTER, WM
本文介绍了一种制备高比放射性（240-300 μ C/μ g）的I ~（131）标记人生长激素的简便、快速的方法。允许少量无载体[I131]碘化物（2 mc）在高产率反应中反应，而无需预先用少量蛋白质（5 [mu]g）处理（约100 μ g）。70%的I131转移至蛋白质）。通过使用无载体的[I131]碘化物使化学取代度最小化（蛋白质的碘分子的0.5-1.0原子）。I131标记的激素（高达300 uC//IG）不含可检测的降解产物，并且与未标记的激素在免疫学上相同。证明了在高比放射性或高水平[I131]碘化学取代时免疫反应性的丧失。
A simple and rapid method is presented for the preparation of I131-labelled human growth hormone of high specific radioactivity (240-300 [mu]C/[mu]g). Low amounts of carrier-free [I131] iodide (2 mc) are allowed to react, without prior treatment with small quantities of protein (5 [mu]g) in a high-yield reaction (approx. 70% transfer of I131 to protein). The degree of chemical substitution is minimized (0.5-1.0 atom of iodine molecule of protein) by the use of carrier-free [I131] iodide. The I131-labelled hormone (up to 300 uC//ig) contains no detectable degradation products and is immunologically identical with the unlabelled hormone. The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with [I131] iodine is demonstrated.