Calcium carbonate mineralization mediated by invitro cultured mantle cells from Pinctada fucata
Calcium carbonate mineralization mediated by invitro cultured mantle cells from Pinctada fucata
复制标题
体外培养的珠母贝套细胞介导的碳酸钙矿化
DOI：
10.1016/j.bbrc.2015.06.057
复制
发表时间：
2015
影响因子：
3.1
通讯作者：
Rongqing Zhang
中科院分区：
文献类型：
--
作者：
Wei Kong;Shiguo Li;Liang Xiang;Liping Xie;Rongqing Zhang
软体动物壳的形成被认为是由基质蛋白介导的细胞外事件。我们报道了由太平洋牡蛎细胞介导的碳酸钙矿化。晶体只出现时，外套细胞存在于结晶溶液中。这些晶体以高度有序的单元堆积，显示出典型的生物矿化产物特征。在将晶体溶解于EDTA中之后观察到薄的有机框架。有些晶体表面被蚀刻，比其他部分光滑得多。观察到外套细胞附着在这些光滑的表面。这些结果表明，外套细胞可能直接参与了碳酸钙矿化的成核和重塑过程。这一结果表明了研究生物成矿作用的套细胞机制的可行性，有助于对壳层形成过程的全面认识。
Formation of the molluscan shell is believed to be an extracellular event mediated by matrix proteins. We report calcium carbonate mineralization mediated byPinctadafucatamantle cells. Crystals only appeared when mantle cells were present in the crystallization solution. These crystals were piled up in highly ordered units and showed the typical characteristics of biomineralization products. A thin organic framework was observed after dissolving the crystals in EDTA. Some crystals had etched surfaces with a much smoother appearance than other parts. Mantle cells were observed to be attached to some of these smooth surfaces. These results suggest that mantle cells may be directly involved in the nucleation and remodeling process of calcium carbonate mineralization. Our result demonstrate the practicability of studying the mantle cell mechanism of biomineralization and contribute to the overall understanding of the shell formation process.