Tumor detection and visualization using cyanine fluorochrome-labeled antibodies
Tumor detection and visualization using cyanine fluorochrome-labeled antibodies
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DOI：
10.1021/bp970088t
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发表时间：
1997-09-01
影响因子：
2.9
通讯作者：
Farkas, DL
中科院分区：
文献类型：
--
作者：
Ballou, B;Fisher, GW;Farkas, DL
随着肿瘤靶向分子，特别是单抗及其衍生物的改进，方便的近红外发射荧光染料的发展，以及在该光谱区域具有高灵敏度的数码相机的出现，利用荧光进行肿瘤定位已经成为现实。最近在动物身上的研究表明，单抗的荧光标记具有足够的灵敏度和更高的分辨率。荧光标记抗体和放射性标记抗体的分布和分解代谢相似。通过用几种不同的近红外发射荧光染料进行标记，可以同时定位多种试剂；因此，可以对多种肿瘤相关成分进行背景减除和差异标记。使用荧光标记的困难主要与组织中的光散射和吸收有关，但检测几毫米深处的小肿瘤是可行的。这项新技术的主要医疗用途很可能是肿瘤的内窥镜定位。科学用途包括研究肿瘤转移、肿瘤对药物和肿瘤靶向分子的摄取和分布，以及近红外标记细胞在体内的迁移模式。
Tumor localization using fluorescence has been made practical by current improvements in tumor targeting molecules, especially monoclonal antibodies and their derivatives, by the development of convenient near-infrared emitting fluorochromes and by the availability of digital cameras having high sensitivity in this spectral region. Recent studies in animals have demonstrated that fluorochrome labeling of monoclonal antibodies confers adequate sensitivity and improved resolution. Distribution and catabolism of fluorochrome-labeled and radiolabeled antibodies are similar. Simultaneous localization of multiple reagents is made possible by labeling with several different near-infrared emitting fluorochromes; thus background subtraction and differential labeling of multiple tumor-associated components can be performed. Difficulties in using the fluorochrome labels are mainly related to light scattering and absorption in tissues, but detection of small tumors at depths of several millimeters is feasible. The major medical use of this new technology is likely to be endoscopic location of tumors. Scientific uses include studies of tumor metastasis, uptake and distribution of drugs and tumor-targeting molecules by tumors, and migration patterns of near-infrared labeled cells in vivo.