Life Extension by Diet Restriction and N-Acetyl-L-Cysteine in Genetically Heterogeneous Mice
Life Extension by Diet Restriction and N-Acetyl-L-Cysteine in Genetically Heterogeneous Mice
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DOI：
10.1093/gerona/glq155
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发表时间：
2010-12-01
影响因子：
5.1
通讯作者：
Harrison, David E.
中科院分区：
文献类型：
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作者：
Flurkey, Kevin;Astle, Clinton M.;Harrison, David E.
我们使用异质小鼠种群——UM-HET3（CByB6F1/J 和 C3D2F1/J 父母的第一代后代）来测试六种抗衰老治疗对寿命的影响。在第一份针对结构化、隔离异质人群的饮食限制报告中，我们观察到与 CByB6F1/J 杂交阳性对照中发现的平均寿命和最大寿命基本相同的增加。我们还报告了在 7 个月时开始使用 N-乙酰基-L-半胱氨酸治疗的结果，以及在 16-18 个月时开始使用阿司匹林、硝基氟比洛芬、4-羟基苯基 N-叔丁基硝酮和去甲二氢愈创木酸的治疗结果。只有接受 N-乙酰基-L-半胱氨酸的雄性 UM-HET3 小鼠的寿命显着延长，这可能是由于与治疗相关的无意的饮食限制。其他药物对寿命没有显着影响。 UM-HET3 小鼠的使用有助于确保这些结果不是单一基因型无反应的结果，而是它们更广泛地代表实验室小鼠。
We used a heterogeneous stock of mice-UM-HET3, the first generation offspring of CByB6F1/J and C3D2F1/J parents-to test effects of six antiaging treatments on life span. In the first report of diet restriction in a structured, segregating heterogeneous population, we observed essentially the same increases in mean and maximum life span as found in CByB6F1/J hybrid positive controls. We also report results of treatment with N-acetyl-L-cysteine started at 7 months, and aspirin, nitroflurbiprofen, 4-hydroxy phenyl N-tert-butyl nitrone, and nordihydroguaiaretic acid, all started at 16-18 months. Only male UM-HET3 mice receiving N-acetyl-L-cysteine had significantly increased life span, and this may have been due to treatment-related inadvertent diet restriction. The other agents had no significant effects on life span. The use of UM-HET3 mice helps assure that these results are not the result of unresponsiveness of a single genotype but that they more broadly represent laboratory mice.