Polyethylene glycol-grafted polystyrene particles
Polyethylene glycol-grafted polystyrene particles
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DOI：
10.1002/jbm.a.30056
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Feijen, J
中科院分区：
文献类型：
--
作者：
Meng, FH;Engbers, GHM;Feijen, J
利用碳二亚胺化学将氨基聚乙二醇（PEG，分子量3400或5000）共价接枝到羧基聚苯乙烯颗粒（PS-COOH）上，制备了密集聚乙二醇颗粒，可作为人工细胞的模型体系。通过测定聚乙二醇表面浓度、c电位、大小和形貌对聚乙二醇修饰颗粒（PS-PEG）进行了表征。在优化的接枝条件下，形成了致密的“刷状”聚乙二醇层。可以实现约60 pmol/cm(2)的PEG表面浓度，对应于接枝PEG链之间的平均距离约为17埃。结果表明，将PEG接枝到PS-COOH上可使人血浆中蛋白质（85 vol %）在磷酸盐缓冲盐水中的吸附率降低90%。(C) 2004 Wiley期刊有限公司
Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, C-potential, size, and morphology. Under optimized grafting conditions, a dense "brush-like" PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm(2), corresponding with an average distance between grafted PEG chains of similar to17 Angstrom can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%. (C) 2004 Wiley Periodicals, Inc.