Semicomprehensive Analysis of the Postnatal Age-Related Changes in the mRNA Expression of Sex Steroidogenic Enzymes and Sex Steroid Receptors in the Male Rat Hippocampus
Semicomprehensive Analysis of the Postnatal Age-Related Changes in the mRNA Expression of Sex Steroidogenic Enzymes and Sex Steroid Receptors in the Male Rat Hippocampus
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DOI：
10.1210/en.2010-0581
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发表时间：
2010-12-01
期刊：
影响因子：
4.8
通讯作者：
Kawato, Suguru
中科院分区：
文献类型：
--
作者：
Kimoto, Tetsuya;Ishii, Hirotaka;Kawato, Suguru
虽然性类固醇激素在出生后的大脑发育中起着至关重要的作用，但海马类固醇激素合成的年龄相关变化在很大程度上仍然未知。采用RT-PCR/Southern杂交技术，对出生后1、4、7、10、14、4 wk和12 wk（成年）雄性大鼠海马26种性类固醇生成酶/蛋白和3种性类固醇受体的mRNA表达进行了研究。这些酶/受体在PD 1中的相对表达水平为Srd 5a 1>星星> Ar类似于Hsd 17 b4类似于Hsd 17 b1类似于Hsd 17 b7类似于Esr 1类似于Srd 5a 2> Hsd 17 b3> Esr 2> Cyp 11 a1> Cyp 17 a1> Cyp 19 a1类似于Hsd 17 b2> 3 β-羟基类固醇脱氢酶I。孕酮/睾酮/雌二醇代谢必需酶（Cyp 17 a1，Hsd 17 b7和Cyp 19 a1）的mRNA水平在PD 1和PD 14之间大致恒定，然后向成人水平下降。Cyp 11 a1在PD 4-PD 14期间增加，然后向成人水平显著降低（与PD 1的8%相似）。Hsd 17 b1、Hsd 17 b2和3 β-羟基类固醇脱氢酶I mRNA大致单调下降。在PD 4-PD 14期间，Hsd 17 b3增加至PD 1的约200%，并维持在该高水平。5 α-还原酶mRNA保持恒定（Srd 5a 1）或单调下降（Srd 5a 2）向成人水平。Esr 1在PD 4时达到峰值，并向成年水平下降，而Ar在PD 1-PD 14期间大幅增加，并维持在这个高水平。从新生儿到成人，星星和Hsd 17 b4水平保持恒定。这些结果表明，海马性类固醇激素的性质在出生后的发展过程中，这可能有助于大脑性成熟的实质性改变。（内分泌学151：5795-5806，2010）
Although sex steroids play a crucial role in the postnatal brain development, the age-related changes in the hippocampal steroidogenesis remain largely unknown. We performed comprehensive investigations for the mRNA expressions of 26 sex steroidogenic enzymes/proteins and three sex steroid receptors in the male rat hippocampus, at the ages of postnatal day (PD) 1, PD4, PD7, PD10, PD14, 4 wk, and 12 wk (adult), by RT-PCR/Southern blotting analysis. The relative expression levels of these enzymes/receptors at PD1 were Srd5a1 > Star > Ar similar to Hsd17b4 similar to Hsd17b1 similar to Hsd17b7 similar to Esr1 similar to Srd5a2 > Hsd17b3 > Esr2 > Cyp11a1 > Cyp17a1 > Cyp19a1 similar to Hsd17b2 > 3 beta-hydroxysteroid dehydrogenase I. The mRNA levels of essential enzymes for progesterone/testosterone/estradiol metabolisms (Cyp17a1, Hsd17b7, and Cyp19a1) were approximately constant between PD1 and PD14 and then declined toward the adult levels. Cyp11a1 increased during PD4-PD14 and then considerably decreased toward the adult level (similar to 8% of PD1). Hsd17b1, Hsd17b2, and 3 beta-hydroxysteroid dehydrogenase I mRNA decreased approximately monotonously. Hsd17b3 increased to approximately 200% of PD1 during PD4-PD14 and was maintained at this high level. The 5 alpha-reductase mRNA was maintained constant (Srd5a1) or decreased monotonically (Srd5a2) toward the adult level. The Esr1 level peaked at PD4 and decreased toward the adult level, whereas Ar greatly increased during PD1-PD14 and was maintained at this high level. The Star and Hsd17b4 levels were maintained constant from neonate to adult. These results suggest that the hippocampal sex steroidogenic properties are substantially altered during the postnatal development processes, which might contribute to brain sexual maturation. (Endocrinology 151: 5795-5806, 2010)