Biocompatible conductive architecture of carbon nanofiber-doped chitosan prepared with controllable electrodeposition for cytosensing
Biocompatible conductive architecture of carbon nanofiber-doped chitosan prepared with controllable electrodeposition for cytosensing
复制标题
可控电沉积制备碳纳米纤维掺杂壳聚糖的生物相容性导电结构用于细胞传感
DOI：
10.1021/ac062344z
复制
发表时间：
2007-06-15
影响因子：
7.4
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Hao, Chen;Ding, Lin;Ju, Huangxian
结合壳聚糖(CS)的生物相容性和纳米碳纤维(CNF)优异的导电性，设计了一种新型结构。通过可控电沉积法制备了一种具有良好生物相容性的CNF-CS纳米复合膜，用于K562细胞在电极上的固定和细胞传感。用扫描电子显微镜、红外光谱、接触角和热重分析对所形成的结构进行了表征。用电化学阻抗谱和循环伏安法研究了K562细胞在纳米复合膜修饰电极上的附着情况。CNF的存在促进了K562细胞的电化学行为。电子转导的阻抗与贴壁细胞的数量有关，为K562细胞提供了一种高灵敏度的阻抗传感器，检测范围为5×10(3)到5.0×10(7)细胞(-1)，检测下限为1×10(3)细胞(-1)。这项工作提出了一种制备生物相容性和导电界面的策略，用于细胞的固定化和电化学检测，为CNF在细胞检测中的应用开辟了一条道路。
A novel architecture was designed by combining the biocompatibility of chitosan (CS) and excellent conductivity of carbon nanofiber (CNF). The controllable electrodeposition of soluble CNF-doped CS colloidal solution formed a robust CNF-CS nanocomposite film with good biocompatibility for the immobilization and cytosensing of K562 cells on an electrode. The formed architecture was characterized using scanning electron microscopic, infrared spectrum, contact angle, and thermogravimetric analyses. The adhesion of K562 cells on the nanocomposite film-modified electrode could be followed with electrochemical impedance spectroscopy and cyclic voltammetry. The presence of CNF facilitated the electrochemical behavior of K562 cells. The impedance of electronic transduction was related to the amount of the adhered cells, producing a highly sensitive impedance sensor for K562 cells ranging from 5 x 10(3) to 5.0 x 10(7) cells mL(-1) with a limit of detection of 1 x 10(3) cells mL(-1). This work suggested a strategy to prepare a biocompatible and conductive interface for immobilization and electrochemical detection of cells and opened a way for the application of CNF in cytosensing.