Possible Recruitment of Peripheral Blood CXCR3+ CD27+ CD19+ B Cells to the Liver of Chronic Hepatitis C Patients
Possible Recruitment of Peripheral Blood CXCR3+ CD27+ CD19+ B Cells to the Liver of Chronic Hepatitis C Patients
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DOI：
10.1089/jir.2009.0047
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发表时间：
2010-04-01
影响因子：
2.3
通讯作者：
Yamaguchi, Kazunari
中科院分区：
文献类型：
--
作者：
Mizuochi, Toshiaki;Ito, Masahiko;Yamaguchi, Kazunari
已经提出丙型肝炎病毒（HCV）不仅感染肝细胞，而且感染免疫细胞，包括B细胞。B细胞的HCV感染可能是B细胞失调性疾病的原因，例如混合性冷球蛋白血症、类风湿因子产生和B细胞淋巴增生性疾病，其可能演变成非霍奇金淋巴瘤。为了阐明慢性HCV感染对B细胞动力学的影响，对慢性丙型肝炎患者（CHC）的外周B细胞进行了表征。我们发现CHC患者外周血中CD 27（+）B细胞的频率，即记忆表型，显著降低。同时，CHC血浆中IFN-γ诱导蛋白-10（IP-10）（CXCR 3配体）的量显著升高。此外，发现CD 27（+）B细胞群在CHC中高度表达CXCR 3，因此表明CD 27（+）B细胞群从外周血募集到CHC肝脏的炎症部位，在那里产生IP-10。肝内淋巴细胞免疫组化染色显示CXCR 3（+）B细胞浸润于CHC肝内。因此，我们的研究结果提供了新的见解记忆B细胞在HCV发病机制中的作用。
It has been suggested that hepatitis C virus (HCV) infects not only hepatocytes but also immune cells, including B cells. HCV infection of B cells is the likely cause of B-cell dysregulation disorders such as mixed cryoglobulinemia, rheumatoid factor production, and B-cell lymphoproliferative disorders that may evolve into non-Hodgkin's lymphoma. To clarify the effects of chronic HCV infection on B-cell dynamics, peripheral B cells from chronic hepatitis C patients (CHC) were characterized. We found that the frequency of CD27(+) B cells, that is memory phenotype, was significantly reduced in the peripheral blood of CHC. At the same time, the amount of IFN-gamma-inducible protein-10 (IP-10), a CXCR3 ligand, was markedly elevated in the plasma of CHC. Furthermore, the CD27(+) B-cell population was found to highly express CXCR3 in CHC, thus suggesting that the CD27(+) B-cell population was recruited from peripheral blood to the inflammatory site of the liver of CHC, where IP-10 is produced. Immunohistochemical analyses of intrahepatic lymphocytes indicated that CXCR3(+) B cells were infiltrated in the liver of CHC. Our results thus offer new insight into the role of memory B cells in the HCV pathogenesis.