A mechanism by which Astragalus polysaccharide protects against ROS toxicity through inhibiting the protein dephosphorylation of boar sperm preserved at 4°C
A mechanism by which Astragalus polysaccharide protects against ROS toxicity through inhibiting the protein dephosphorylation of boar sperm preserved at 4°C
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DOI：
10.1002/jcp.26321
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发表时间：
2018-07-01
影响因子：
5.6
通讯作者：
Li, Xinhong
中科院分区：
文献类型：
--
作者：
Fu, Jieli;Yang, Qiangzhen;Li, Xinhong
大量研究表明，黄芪多糖（APS）具有较强的抗氧化作用，对精液体外低温保存具有较高的实用价值。然而，迄今为止，很少有人关注APS在4 ℃下保存精子的确切机制。因此，为了进一步了解APS的保护作用，本研究进行了评估精子质量参数，抗氧化能力，ATP含量和蛋白磷酸化水平的变化。在这里，我们证明了补充APS可以有效地保护公猪精子质量参数，如精子活力，顶体完整性和线粒体膜电位。APS对公猪精子质量的影响主要是通过清除线粒体中过量的ROS、提高抗氧化能力和提高ATP水平来实现的。有趣的是，通过对蛋白磷酸化的一系列研究，我们还发现APS可以通过cAMP-PKA信号通路抑制ROS引起的蛋白去磷酸化，从而保护猪精子免受氧化应激和能量缺乏的影响。据我们所知，这是第一次从能量代谢和蛋白质修饰的角度探索APS对ROS毒性保护作用的分子机制。本研究全面揭示了抗氧化剂对4 ℃保存精子的保护作用的作用机制，并揭示了APS作为辅助生殖技术的公猪精液补充剂的实际可行性。
Numerous studies have shown that Astragalus polysaccharide (APS) has strong antioxidant effects and high practical value for preserving semen at low temperatures in vitro. However, to date, little attention has been paid to the precise mechanism of APS in sperm preservation at 4 degrees C. Thus, to gain further insight into the protective effects of APS, the present study was performed to assess the changes in sperm quality parameters, antioxidant capacity, ATP content, and protein phosphorylation levels. Here, we demonstrated that supplementation with APS could effectively preserve boar sperm quality parameters such as sperm motility, acrosome integrity, and mitochondrial membrane potential. Moreover, we found that the positive effects of APS on boar sperm quality were mainly due to the elimination of excessive mitochondrial ROS, the improvement of antioxidant capacities and the enhancement of ATP levels. Interestingly, by conducting a series of studies on protein phosphorylation, we also discovered that APS could protect boar sperm from oxidative stress and energy deficiency through inhibiting the protein dephosphorylation caused by ROS via the cAMP-PKA signaling pathway. To our knowledge, this is the first exploration of the molecular mechanism underlying the protective roles of APS toward ROS toxicity from the perspective of energy metabolism and protein modification. This study comprehensively provides novel insights into the action mechanism of the protective effects of antioxidants on sperm stored at 4 degrees C and reveals the practical feasibility of using APS as a boar semen extender supplement for assisted reproductive technology.