Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex
Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex
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作者：
Fern치ndez-Espinar, MT;Barrio, E;Querol, A
采用随机扩增多态性dna -聚合酶链反应（RAPD-PCR）方法对构成严格感觉酵母菌复体最常见菌株的兄弟种baycharomyces、酿酒酵母菌（s.s cerevisiae）、悖论酵母菌（s.s paradoxus）和巴斯德酵母菌（s.s pastorianus）进行了鉴定。用任意序列的6个十聚体引物扩增了58个菌株的DNA。每个物种都获得了物种特异性（诊断）带。通过相邻连接法和最大简约法构建的两种系统发育树清楚地表明，利用RAPD分析可以区分这些亲缘种。得到4组菌株，分别对应bayanus s、酿酒s、paradox s和pastorianus s。在S. bayanus分类群中，观察到两组菌株。其中一株包括前一种葡萄球菌型菌株CECT1969T和与其密切相关的葡萄菌株（葡萄变种葡萄球菌），另一株包括葡萄球菌型菌株CECT1941(T)和菌株CECT1991和10513（葡萄变种葡萄球菌）。异种悖论葡萄球菌类群可划分为3个谱系，分别对应于不同的地理起源，即美洲、日本和欧洲人群。此外，由于RAPD-PCR标记的多位点特性，该方法对于酵母杂交来源的鉴定是有用且合适的。通过分析各杂交品系与亲本共有的条带比例，推断其杂交性质。在分析的58株菌株中，有6株是杂交菌株，尽管来自每个亲本的基因组比例因菌株而异。版权所有：John Wiley Sons， Ltd。
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was applied to differentiate the sibling species Saccharomyces bayanus, S. cerevisiae, S. paradoxus and S. pastorianus, which constitute the most common strains of the Saccharomyces sensu stricto complex. Six decamer primers of arbitrary sequences were used to amplify the DNA of 58 strains. Species-specific (diagnostic) bands were obtained for each species. Two phylogenetic trees constructed by the neighbour-joining and maximum parsimony methods clearly showed that the delimitation of these related Yeast species is possible by using RAPD analysis. Four groups of strains, corresponding to the species S. bayanus, S. cerevisiae, S. paradoxus and S. pastorianus, were obtained. Within the S. bayanus taxon, two groups of strains were observed. One includes the former type strain of S. uvarum, CECT1969T, and closely related wine strains (S. bayanus var. uvarum), whilst the other contains S. bayanus type strain CECT1941(T) and strains CECT1991 and 10513 (S. bayanus var. bayanus). The heterogeneous S. paradoxas group was divided into three lineages, corresponding to different geographic origin, American, Japanese and European populations. In addition, due to the multilocus nature of the RAPD-PCR marker, this method is both useful and appropriate for the identification of the hybrid origin of S. pastorianus. The hybrid nature was deduced from the analysis of the fraction of bands shared by each hybrid strain and the parental species. Among the 58 strains analysed, six S. pastorianus strains were hybrids, although the fraction of genome coming from each parent varied depending on the strain. Copyright (C) 2003 John Wiley Sons, Ltd.