The ubiquitin-binding protein MdRAD23D1 affects WUE under long-term moderate drought stress in transgenic apple (Malus domestica)
The ubiquitin-binding protein MdRAD23D1 affects WUE under long-term moderate drought stress in transgenic apple (Malus domestica)
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DOI：
10.1016/j.scienta.2023.112164
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发表时间：
2023-09
影响因子：
4.3
通讯作者：
Xiaoli Zhang;Xiaoqing Gong;Benzhou Zhao;Jingwen Huang;Hongyu Zhou;Mingjun Li;F. Ma
中科院分区：
文献类型：
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作者：
Xiaoli Zhang;Xiaoqing Gong;Benzhou Zhao;Jingwen Huang;Hongyu Zhou;Mingjun Li;F. Ma
水分亏缺是苹果生长和生产力面临的挑战，提高水分利用效率对苹果植物适应干旱气候具有重要意义。RAD23(Radiation SENSITIVE23)蛋白是一组UBL-UBA(泛素样泛素相关)蛋白，它将泛素化的蛋白质穿梭到26S蛋白酶体上进行分解。在此，我们探讨了苹果Ubl-Uba基因MdRAD23D1在长期中度干旱条件下调节水分利用效率的生物学功能。结果表明，在长期中度干旱条件下，MdRAD23D1-RNAi(RNA干扰抑制MdRAD23D1RNAi)苹果植株较小，水分利用效率较低。在长期中度干旱处理下，RNAi植株的生物量积累和相对含水量低于野生型植株。与WT相比，RNAi植株的根系较小，根系鲜重和干重较低，根系活力和导水率较低。RNAi植株的光合作用能力下降，光系统受到的损害比WT植株严重得多。在长期中度干旱下，RNAi植株积累了更多的活性氧，超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性较低。此外，MdRAD23D1-RNAi苹果植株表现出较高的脱落酸含量，气孔开度受干旱胁迫的影响较大。RNAi植株的游离氨基酸含量低于WT植株。综上所述，我们的结果表明，抑制MdRAD23D1的表达降低了长期干旱胁迫下苹果植株的WUE，这可能是通过影响光合作用效率、气孔行为和氨基酸的积累来实现的。
Water deficit is a challenge for apple (Malus domestica) growth and productivity worldwide, and improving water use efficiency (WUE) is important for apple plants to adapt to arid climates. RAD23 (RADIATION SENSITIVE23) proteins are a group of UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins that shuttle ubiquitylated proteins to the 26S proteasome for breakdown. Here, we explored the biological function of a UBL-UBA geneMdRAD23D1from apple in regulating WUE under long-term moderate drought. The results showed thatMdRAD23D1-RNAi (suppressingMdRAD23D1by RNA interference) apple plants were smaller, and had lower WUE under long-term moderate drought. When treated with long-term moderate drought, the RNAi plants exhibited less biomass accumulation and relative water content than WT (wild type) plants. The roots of RNAi plants were smaller, the roots fresh and dry weights of RNAi plants were lower, as well as lower root activity and hydraulic conductivity compared with WT. Photosynthetic capacity decreased and the photosystems were much more damaged in RNAi plants than in WT. The RNAi plants also accumulated more reactive oxygen species and had lower activities of superoxide dismutase, peroxidase and catalase under long-term moderate drought. In addition,MdRAD23D1-RNAi apple plants showed higher ABA (abscisic acid) contents and stomatal apertures were more affected by drought stress. The free amino acid contents were lower in RNAi plants than in WT. In summary, our results indicated that suppressingMdRAD23D1expression decreased WUE in apple plants under long-term drought stress, possibly by affecting photosynthetic efficiency, stomatal behavior, and accumulation of amino acids.