Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X
Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X
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DOI：
10.1007/s11262-005-2205-z
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发表时间：
2005-08-01
期刊：
影响因子：
1.6
通讯作者：
Namba, S
中科院分区：
文献类型：
--
作者：
Komatsu, K;Kagiwada, S;Namba, S
为了阐明马铃薯X病毒（PVX）株系在宿主植物中引起不同反应的基因组决定簇，我们测定了四个日本PVX株系：PVX-BS，-BH，-OG和-TO的完整基因组RNA序列。这四个菌株，加上先前测序的PVX-OS菌株，在野生马铃薯（Solanum demissum）和烟草（Nicotiana tabacum cv. Samsun NN）。这五种PVX菌株的基因组序列是高度同源的（即，核苷酸序列同一性范围为95.4 - 98.5%）。系统发育分析表明，日本PVX菌株起源于欧洲组的一个祖先PVX菌株，这些菌株在两个S. demissum和烟草的致病性与它们的系统发育关系无关，这表明这些宿主植物中每个菌株的致病性由相对少量的核苷酸决定，并且可以容易地独立于系统发育关系而改变。特别是，OS，BH和BS，分别产生明显对比的环斑，花叶，和无症状感染的烟叶，是最密切相关的，这表明这三个菌株是一个有吸引力的模型，用于分析遗传决定因素引起这些症状。这些菌株的基因组和生物学差异之间可能存在的相关性进行了讨论。
To elucidate the genomic determinants of Potato virus X (PVX) strains, which cause diverse responses in host plants, we determined the complete genomic RNA sequences of four Japanese PVX strains: PVX-BS, -BH, -OG, and -TO. These four strains, plus the previously sequenced PVX-OS strain, differ in their pathogenicity in wild potato (Solanum demissum) and tobacco (Nicotiana tabacum cv. Samsun NN). The genomic sequences of these five PVX strains were highly homologous (i.e., the nucleotide sequence identity ranged from 95.4 to 98.5%). Phylogenetic analysis indicated that the Japanese PVX strains originated from an ancestral PVX strain in the European group, and that the virulence of these strains in both S. demissum and tobacco is not correlated with their phylogenetic relationships, suggesting that the pathogenicity of each strain in these host plants is determined by a relatively small number of nucleotides and can easily be altered independent of phylogenetic relationships. Particularly, OS, BH, and BS, which respectively produce markedly contrasting ringspot, mosaic, and asymptomatic infections in tobacco leaves, were the most closely related, suggesting that these three strains are an attractive model for analyzing the genetic determinants causing these symptoms. A possible correlation between the genomic and biological differences of these strains is discussed.