Critical role of matrix metalloproteinase-9 in acute cold exposure-induced stroke in renovascular hypertensive rats.
Critical role of matrix metalloproteinase-9 in acute cold exposure-induced stroke in renovascular hypertensive rats.
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DOI：
10.1016/j.jstrokecerebrovasdis.2013.05.016
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
Chun-guang Li;Xiang-pen Li;Qingyu Shen;Yi Li;Lei He;Mei Li;Yamei Tang;Yidong Wang;Qingyu He;Ying Peng
中科院分区：
文献类型：
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作者：
Chun-guang Li;Xiang-pen Li;Qingyu Shen;Yi Li;Lei He;Mei Li;Yamei Tang;Yidong Wang;Qingyu He;Ying Peng
Our objectives are to investigate the role of MMP-9 in cold exposure–induced stroke and assess the preventive effect of doxycycline, a total of 200 rats were assigned to a control group, sham group, 2-kidney, 2-clip (2K–2C) group, and doxycycline-received 2K-2C group (2K–2C + doxy) (N = 50, each), and subsequently, each group were randomly assigned to 2 groups: acute cold exposure (ACE) and nonacute cold exposure (NACE) (N = 25, each). After the blood pressure was stabilized, rats were maintained on a 12-h light (22°C)/dark (4°C) cycle (ACE group) or a 12-h light (22°C)/dark (22°C) cycle (NACE group) for 3 cycles. The results showed that ACE upregulated Ang II and MMP-9 protein levels in brains and aortas and considerably enhanced stroke incidence in 2K–2C rats. In contrast, doxycycline treatment prevented upregulation of MMP-9 protein expression and activity in brains and aortas in response to ACE and significantly decreased stroke incidence. These findings suggest that cold exposure–induced MMP-9 via activation of RAS might play a critical role in the initiation of cold exposure–induced stroke during chronic hypertension and doxycycline shows protective effects against cold exposure–induced stroke.
