Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
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DOI：
10.1016/j.biomaterials.2003.10.033
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Khademhosseini, A;Suh, KY;Langer, R
描述了一种用于图案化细胞共培养物的新方法，其使用离子生物聚合物的逐层沉积。使用非生物污染透明质酸（HA）微图案将细胞和蛋白质粘附到玻璃基底上。随后的离子吸附的聚-L-赖氨酸（PLL）的HA模式被用来切换的HA表面从细胞排斥粘附，从而促进第二种细胞类型的粘附。证明了这种方法用于肝细胞或胚胎干细胞与成纤维细胞的模式共培养的实用性。此外，证明了这种方法的通用性，以产生图案化的共培养物，而不管原代细胞接种和接种细胞的相对粘附。因此，所提出的方法可能是一个有用的工具，用于制造受控的细胞共培养的细胞-细胞相互作用的研究和组织工程应用。(C)2003爱思唯尔有限公司。保留所有权利。
A novel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Non-biofouling hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-L-lysine (PLL) to HA patterns was used to switch the HA surfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell-cell interaction studies and tissue engineering applications. (C) 2003 Elsevier Ltd. All rights reserved.