INTRA-THYMIC AND EXTRA-THYMIC EXPRESSION OF THE PRE-T CELL-RECEPTOR ALPHA-GENE
INTRA-THYMIC AND EXTRA-THYMIC EXPRESSION OF THE PRE-T CELL-RECEPTOR ALPHA-GENE
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DOI：
10.1002/eji.1830250713
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发表时间：
1995-07-01
影响因子：
5.4
通讯作者：
RODEWALD, HR
中科院分区：
文献类型：
--
作者：
BRUNO, L;ROCHA, B;RODEWALD, HR
我们分析了来自不同解剖部位的细胞中的前T细胞受体α (pT α)基因表达，以研究pT α RNA的谱系特异性及其在前T细胞和胸腺外T细胞发育部位的存在。pT α RNA存在于α β T细胞的前体中，但不存在于成熟的α β T细胞以及在细胞表面表达γ δ T细胞受体的T细胞中。在成熟和未成熟的B细胞、髓细胞、NK细胞和多能干细胞中，pT α的表达是精确的T谱系特异性的。另一方面，在胸腺外的前T细胞以及T细胞发育的胸腺内和胸腺外部位发现了pT α的表达。后一项发现与胸腺内外T细胞发育的早期步骤可能相似的观点是一致的。
We have analyzed pre-T cell receptor alpha(pT alpha) gene expression in cells from various anatomical sites to investigate the lineage specificity of pT alpha RNA as well as its presence in pro-T cells and in sites of extrathymic T cell development. pT alpha RNA is found in precursors of alpha beta T cells but is absent from mature alpha beta T cells as well as T cells that express the gamma delta T cell receptor on the cell surface. pT alpha expression is exquisitely T lineage specific in that mature and immature B cells, myeloid cells, NK cells and pluripotent stem cells are pT alpha negative. On the other hand, pT alpha expression is found in pro-T cells outside the thymus as well as in intra- and extra-thymic sites of T cell development. The latter finding is consistent with the notion that early steps of T cell development within and outside the thymus may be similar.