Impaired mitochondrial function in murine oocytes is associated with controlled ovarian hyperstimulation and in vitro maturation
Impaired mitochondrial function in murine oocytes is associated with controlled ovarian hyperstimulation and in vitro maturation
复制标题
小鼠卵母细胞线粒体功能受损与受控卵巢过度刺激和体外成熟有关
DOI：
10.1071/rd11212
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发表时间：
2012-01-01
影响因子：
1.9
通讯作者：
Dong, Qiaoxiang
中科院分区：
文献类型：
--
作者：
Ge, Hongshan;Tollner, Theodore L.;Dong, Qiaoxiang
本研究旨在确定控制性超排卵（COH）和体外成熟（IVM），人类IVF治疗中的两种常见临床程序，是否对卵母细胞中线粒体DNA（mtDNA）拷贝数和线粒体功能产生影响。小鼠卵母细胞经COH、IVM和自然周期（NC）后回收成熟卵母细胞，模拟人类临床IVF治疗中的这些处理。测定卵母细胞线粒体DNA的拷贝数、线粒体内膜电位和三磷酸腺苷（ATP）含量测定的线粒体活性、线粒体分布模式、活性氧（ROS）水平和细胞骨架的完整性。COH组和NC组在所有指标中均存在显著差异，但线粒体分布模式和ROS水平除外。IVM处理组和NC处理组的卵母细胞线粒体DNA拷贝数、ROS水平和细胞骨架完整性也存在显著差异。总之，本研究的结果表明，非生理性COH和IVM处理抑制mtDNA复制，改变线粒体功能，增加异常细胞骨架和ROS产生的百分比。与线粒体相关的损伤可能部分解释了IVF的低效率和与这些临床程序相关的高胚胎丢失率。
The present study was designed to determine whether controlled ovarian hyperstimulation (COH) and in vitro maturation (IVM), two common clinical procedures in human IVF treatment, have an impact on mitochondrial DNA (mtDNA) copy number and mitochondrial function in oocytes. Matured mouse oocytes recovered following COH, IVM and natural cycles (NC), which simulated those treatments in human clinic IVF treatment. The copies of mtDNA, the activity of mitochondria as determined by inner mitochondrial membrane potential and oocyte adenosine trisphosphate (ATP) content, pattern of mitochondrial distribution, reactive oxygen species (ROS) levels and the integrity of the cytoskeleton were evaluated in oocytes. Significant differences were detected between COH and NC groups in all measures, except the pattern of mitochondrial distribution and ROS levels. There were also significant differences detected between IVM and NC treatment groups in the copies of mitochondrial DNA, the level of ROS and the integrity of the cytoskeleton in oocytes. In conclusion, the results of this investigation indicate that non-physiological COH and IVM treatments inhibit mtDNA replication, alter mitochondrial function and increase the percentage of abnormal cytoskeleton and ROS production. Damage related to the mitochondria may partly explain the low efficiency of IVF and high rate of embryonic loss associated with these clinical procedures.