Cloning and sequence analysis of a p40 structural protein gene of Helicoverpa zea nuclear polyhedrosis virus.
Cloning and sequence analysis of a p40 structural protein gene of Helicoverpa zea nuclear polyhedrosis virus.
复制标题
玉米夜蛾核型多角体病毒p40结构蛋白基因的克隆及序列分析。
DOI：
10.1006/viro.1993.1025
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Fraser,MJ
中科院分区：
文献类型：
--
作者：
Ma,SW;Corsaro,BG;Klebba,PE;Fraser,MJ
A gene encoding an occluded virion structural protein was isolated from an expression library constructed from theHelicoverpa zeaS-type nuclear polyhedrosis virus (NPV) isolate HzS-15 using both polyclonal and monoclonal antibody screening. The gene was located within aPstI-SalI fragment of the HzS-15 genome spanning from 96.5 to 97.3 m.u. Sequencing analyses revealed a long open reading frame of 927 nucleotides that predicted a protein of 37 kDa in size. Immunoblot analyses using the monoclonal antibody ENV409 demonstrated that the gene corresponded to a 40-kDa protein (p40) in SDS-polyacrylamide gels that was present exclusively in enveloped occluded virions but not in extracellular budded virions or envelope-stripped nucleocapsids. A p40 protein-specific transcript was detected at 16 hr postinfection in HzS-15-infected HZ 1075/UND-K cells and remained until 22 hr. Primer extension analyses demonstrated that the p40 protein-specific transcript started at -49 nucleotides from the ATG start codon within an ATAAG consensus pentamer found in late protein genes of Baculoviruses. The deduced amino acid sequence of the HzS-15 p40 protein gene shared 44 and 45% sequence homology with the p40 proteins ofBombyx moriNPV andAutographa californicaNPV, respectively.
