CONSTRUCTION AND CHARACTERIZATION OF A PROTECTIVE ANTIGEN-DEFICIENT BACILLUS-ANTHRACIS STRAIN
CONSTRUCTION AND CHARACTERIZATION OF A PROTECTIVE ANTIGEN-DEFICIENT BACILLUS-ANTHRACIS STRAIN
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DOI：
10.1111/j.1365-2958.1990.tb00685.x
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发表时间：
1990-07-01
影响因子：
3.6
通讯作者：
MOCK, M
中科院分区：
文献类型：
--
作者：
CATALDI, A;LABRUYERE, E;MOCK, M
将编码炭疽芽孢杆菌三种毒素成分之一的保护性抗原（PA）的pag基因克隆到可移动穿梭载体pAT187中，通过大肠杆菌偶联转移到炭疽芽孢杆菌中。利用这种策略，在大肠杆菌中构建并鉴定了一个插入突变的pag基因，并将其引入炭疽芽孢杆菌菌株。利用该转偶联物选择了一个重组克隆（RP8），该克隆在毒素编码质粒pX01上携带失活的pag基因。菌株RP8缺乏PA，但仍产生另外两种毒素成分，即致死因子（LF）和水肿因子（EF）。与野生型斯特恩菌株的孢子相比，RP8制备的孢子在小鼠中完全不致死。这些结果清楚地确立了PA在炭疽芽孢杆菌致病性中的核心作用。
The pag gene coding for protective antigen (PA), one of the three toxin components of Bacillus anthracis, has been cloned into the mobilizable shuttle vector pAT187 and transferred by conjugation from Escherichia coli to B. anthracis. Using this strategy, an insertionally mutated pag gene constructed and characterized in E. coli, was introduced into B. anthracis Sterne strain. This transconjugant was used to select a recombinant clone (RP8) carrying the inactivated pag gene on the toxin-encoding plasmid, pX01. Strain RP8 was deficient for PA while still producing the two other toxin components, i.e. lethal factor (LF) and edema factor (EF). In contrast to spores from the wild-type Sterne strain, spores prepared from RP8 were totally non-lethal in mice. These results clearly establish the central role played by PA in B. anthracis pathogenicity.