Early gene expression profile in mouse brain after exposure to ionizing radiation
Early gene expression profile in mouse brain after exposure to ionizing radiation
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DOI：
10.1667/rr3485.1
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Wong, CS
中科院分区：
文献类型：
--
作者：
Mahmoud-Ahmed, AS;Atkinson, S;Wong, CS
利用基因芯片技术研究了电离辐射照射后小鼠脑内基因表达谱的急性变化。在20戈伊照射后0.25、1、5和24 h以及2或10戈伊照射后5 h分离RNA。将RNA杂交到15 K cDNA微阵列上，并使用GeneSpring和Significant分析数据。微阵列分析。20戈伊照射后0.25、1、5和24 h分别有128、334、325和155个基因和ESTs表达受到辐射调制; 2和10戈伊照射后5 h分别有60和168个基因和ESTs表达受到辐射调制。表达谱显示差异调节基因和EST的表达水平和数量的剂量和时间依赖性变化。78个基因被调节了两次或更多次。差异调节基因与12种不同类型的分子功能和24种不同的生物学途径相关，并显示出时间和剂量依赖性变化。使用定量实时PCR验证辐照后四个基因（Jak 3，Dffb，Nsep 1和Terf 1）的表达变化。在另一种小鼠品系中观察到Jak 3的上调。在小鼠脑中，照射后Jak 3免疫反应性增加。总之，照射后大脑中基因谱的变化是复杂的，并依赖于时间和剂量，具有不同功能和途径的基因受到调制。(c)2006年，辐射研究协会。
Acute changes in the gene expression profile in mouse brain after exposure to ionizing radiation were studied using microarray analysis. RNA was isolated at 0.25, 1, 5 and 24 h after exposure to 20 Gy and at 5 h after exposure of the whole brain of adult mice to 2 or 10 Gy. RNA was hybridized onto 15 K cDNA microarrays, and data were analyzed using GeneSpring and Significant. Analysis of Microarray. Radiation modulated the expression of 128, 334, 325 and 155 genes and ESTs at 0.25, 1, 5 and 24 h after 20 Gy and 60 and 168 at 5 h after 2 and 10 Gy, respectively. The expression profiles showed dose- and time-dependent changes in both expression levels and numbers of differentially modulated genes and ESTs. Seventy-eight genes were modulated at two or more times. Differentially modulated genes were associated with 12 different classes of molecular function and 24 different biological pathways and showed time- and dose-dependent changes. The change in expression of four genes (Jak3, Dffb, Nsep1 and Terf1) after irradiation was validated using quantitative real-time PCR. Up-regulation of Jak3 was observed in another mouse strain. In mouse brain, there was an increase of Jak3 immunoreactivity after irradiation. In conclusion, changes in the gene profile in the brain after irradiation are complex and are dependent on time and dose, and genes with diverse functions and pathways are modulated. (c) 2006 by Radiation Research Society.