Differential modulation of adenylyl cyclases I and II by various Gβ subunits
Differential modulation of adenylyl cyclases I and II by various Gβ subunits
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DOI：
10.1074/jbc.273.4.2273
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发表时间：
1998-01-23
影响因子：
4.8
通讯作者：
Vogel, Z
中科院分区：
文献类型：
--
作者：
Bayewitch, ML;Avidor-Reiss, T;Vogel, Z
关于G.（β-γ）二聚体指出，AC的各种亚型对游离G的存在有不同的反应，（β-γ）已经证明，AC I活性受到抑制，AC II活性受到G这个结果并没有解决可能存在于不同的G亚基之间的调节中的可能的G差异。迄今为止，已经克隆了6种G（β）亚型和12种G（γ）亚型。我们已经建立了一种细胞转染系统，其中G（β）和G（γ）cDNA与AC亚型I或II共转染，并测定这些亚型的活性，我们发现，虽然AC I活性被G（β 1/γ 2）和G（β 5/γ 2）组合抑制，但AC II的反应不同，并被G（β 1/γ 2）刺激。这一发现证明了G（β-γ）的不同组合对相同AC亚型的差异调节活性，并证明了AC信号传导系统内的另一水平的复杂性。
The accepted dogma concerning the regulation of adenylyl cyclase (AC) activity by G(beta gamma) dimers states that the various isoforms of AC respond differently to the presence of free G(beta gamma) It has been demonstrated that AC I activity is inhibited and AC II activity is stimulated by G(beta gamma) subunits, This result does not address the possible G differences in modulation that may exist among the different G(beta gamma) heterodimers, Six isoforms of G(beta) and 12 isoforms of G(gamma) have been cloned to date, We have established a cell transfection system in which G(beta) and G(gamma) cDNAs were cotransfected with either AC isoform I or II and the activity of these isoforms was determined, We found that while AC I activity was inhibited by both G(beta 1/gamma 2) and G(beta 5/gamma 2) combinations, AC II responded differentially and was stimulated by G(beta 1/gamma 2). This finding demonstrates differential modulatory activity by different combinations of G(beta gamma) on the same AC isoform and demonstrates another level of complexity within the AC signaling system.