Messenger RNA for ribosomal proteins in Xenopus laevis oocytes.
Messenger RNA for ribosomal proteins in Xenopus laevis oocytes.
复制标题
非洲爪蟾卵母细胞中核糖体蛋白的信使 RNA。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
E. Beccari
中科院分区：
文献类型：
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作者：
P. Pierandrei;E. Beccari
非洲爪蟾卵母细胞（II--III 期）将 [35S] 蛋氨酸掺入可通过二维电泳识别的核糖体蛋白中。从该来源制备的富含 Poly(A) 的 RNA 已在小麦胚芽无细胞系统中进行翻译。在体外合成的产物中，有 30 种可以清楚地识别为核糖体蛋白。有些不能单独解决，而其中一些可能缺乏蛋氨酸或在体外合成后可能无法正确处理。我们的核糖体蛋白活性 mRNA 制剂的富集可以通过在寡 (dT)-纤维素柱上进行后续传代以及通过蔗糖梯度沉降选择 10--16-S 级分来获得。我们估计核糖体蛋白的信使 RNA 占该 RNA 制剂的 10--20%，现在可用于分子克隆程序。
Xenopus laevis oocytes (stage II--III) incorporate [35S]methionine into ribosomal proteins identifiable by two-dimensional electrophoresis. Poly(A)-rich RNA prepared form this source has been translated in a wheat germ cell-free system. Among the products synthesized in vitro 30 are clearly identifiable as ribosomal proteins. Some are not individually resolved, whereas a few of them may lack methionine or may not be correctly processed after the synthesis in vitro. An enrichment of our mRNA preparation for ribosomal protein activity can be obtained by subsequent passages on oligo(dT)-cellulose columns and by selection of a 10--16-S fraction by sucrose gradient sedimentation. We estimate that messenger RNA for ribosomal proteins represents 10--20% of this RNA preparation and can now be utilized for molecular cloning procedures.