Levels of interleukin-8 in tuberculous pleurisy and the profile of immunocompetent cells in pleural and peripheral compartments
Levels of interleukin-8 in tuberculous pleurisy and the profile of immunocompetent cells in pleural and peripheral compartments
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DOI：
10.1016/s0165-2478(96)02649-1
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发表时间：
1997-01-01
影响因子：
4.4
通讯作者：
Bottasso, O
中科院分区：
文献类型：
--
作者：
Dlugovitzky, D;Rateni, L;Bottasso, O
我们的研究调查了13例结核病患者胸膜渗出液中IL-8的存在，并评估其是否与其胸膜和外周室中存在的主要免疫活性细胞的特征有关。为了进行比较，纳入了另一组伴胸膜炎性胸腔积液（PNE）的患者（n = 7）。外周血免疫表型的研究也进行了12个年龄匹配的健康对照组（合作），和39例肺结核患者分类，根据肺受累的程度，分为轻度（n = 9）和先进（n = 30）的情况下。患者在开始治疗前招募，具有HIV阴性血清学，并且在组间没有显示年龄差异（平均值+/- S.D.，40.7+/- 14.7岁）。通过ELISA方法测量IL-8浓度，同时通过使用与以下细胞表面分子反应的FITC缀合的单克隆抗体进行免疫表型分析：CD 3、CD 4、CD 8、CD 25（IL-2 R（+）细胞）、CD 19和CD 68。在所有胸腔渗出液中均检测到IL-8，但TB患者的水平（384 +/- 110 pg/ml）明显高于PNE组（185 +/- 110 pg/mg）（P
Our study investigated the presence of IL-8 in pleural exudates from tuberculosis patients (TBP (n = 13), and evaluated whether it was related with the profile of major immunocompetent cells present in their pleural and peripheral compartments. To allow comparisons, an additional group of patients with parapneumonic pleural effusions (PNE) (n = 7) was included. Blood peripheral immunophenotypic studies were also carried out in 12 age-matched healthy controls (Co), and 39 tuberculosis patients classified, according to the extent of pulmonary involvement, into mild (n = 9), and advanced (n = 30) cases. Patients were recruited before starting therapy, had HIV negative serology, and showed no age differences among groups (mean +/- S.D., 40.7 +/- 14.7 years). IL-8 concentrations were measured by an ELISA method while immunophenotypic analysis was performed by using FITC-conjugated monoclonal antibodies reacting against the following cell surface molecules: CD3, CD4, CD8, CD25 (IL-2R(+) cells), CD19, and CD68. IL-8 was detected in all pleural exudates though levels in the TB patients, 384 +/- 110 pg/ml, appeared significantly higher than the PNE group, 185 +/- 110 pg/mg, (P