Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms
Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms
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DOI：
10.1016/j.plaphy.2011.03.012
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发表时间：
2011-10-01
影响因子：
6.5
通讯作者：
Martinez, Manuel
中科院分区：
文献类型：
--
作者：
Carrillo, Laura;Herrero, Ignacio;Martinez, Manuel
来自植物的蛋白酶抑制剂参与了对害虫和病原体的防御机制。植物胱抑素和胰蛋白酶/ α -淀粉酶抑制剂是植物中两个最具代表性的蛋白酶抑制剂家族。在大麦中，已有13种胱抑素（HvCPI-1至13）和BTI-CMe胰蛋白酶抑制剂的研究。它们抑制害虫消化蛋白酶的能力，以及表达这些抑制剂的植物对害虫的体内负作用，支持了这些蛋白质的防御功能。大麦胱抑素也能抑制体外真菌生长。然而，这些抑制剂在体内的抗真菌作用尚未得到验证。此外，它们在体内和体外对植物病原菌的作用尚不清楚。为了获得这一特性的新见解，我们利用胰蛋白酶抑制剂BTI-CMe和13种大麦胱抑素对植物不同细菌和真菌病原体进行了体外实验。大多数大麦胱抑素和BTI-CMe抑制剂能够抑制菌丝生长，但不能抑制细菌生长。研究了独立表达BTI-CMe抑制剂和胱抑素HvCPI-6的转基因拟南芥植株对相同细菌和真菌病原体的抗性。表达HvCPI-6的转基因植株和BTI-CMe的转基因植株对植物病原真菌和细菌的抗性均不高于对照拟南芥植株。本文讨论了植物病原菌在体外和植物体内测定的差异。(C) 2011 Elsevier Masson SAS。版权所有。
Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/alpha-amylase inhibitors are two of the best characterized protease inhibitor families in plants. In barley, thirteen cystatins (HvCPI-1 to 13) and the BTI-CMe trypsin inhibitor have been previously studied. Their capacity to inhibit pest digestive proteases, and the negative in vivo effect caused by plants expressing these inhibitors on pests support the defence function of these proteins. Barley cystatins are also able to inhibit in vitro fungal growth. However, the antifungal effect of these inhibitors in vivo had not been previously tested. Moreover, their in vitro and in vivo effect on plant pathogenous bacteria is still unknown. In order to obtain new insights on this feature, in vitro assays were made against different bacterial and fungal pathogens of plants using the trypsin inhibitor BTI-CMe and the thirteen barley cystatins. Most barley cystatins and the BTI-CMe inhibitor were able to inhibit mycelial growth but no bacterial growth. Transgenic Arabidopsis plants independently expressing the BTI-CMe inhibitor and the cystatin HvCPI-6 were tested against the same bacterial and fungal pathogens. Neither the HvCPI-6 expressing transgenic plants nor the BTI-CMe ones were more resistant to plant pathogen fungi and bacteria than control Arabidopsis plants. The differences observed between the in vitro and in planta assays against phytopathogenic fungi are discussed. (C) 2011 Elsevier Masson SAS. All rights reserved.