Coexpression of ephrin-Bs and their receptors in colon carcinoma
Coexpression of ephrin-Bs and their receptors in colon carcinoma
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DOI：
10.1002/cncr.10122
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发表时间：
2002-02-15
期刊：
影响因子：
6.2
通讯作者：
Ellis, LM
中科院分区：
文献类型：
--
作者：
Liu, WB;Ahmad, SA;Ellis, LM
背景产生红细胞生成素的肝癌扩增序列（Eph）家族是受体酪氨酸激酶（RTK）的最大亚家族。Eps（受体）与特定的细胞结合配体（称为ephrin）结合。这种配体-受体系统的结合依赖于细胞-细胞相互作用。ephrin-Eph系统在胚胎发育和神经及血管系统的分化中起重要作用。在目前的研究中，作者假设ephrin可能在结肠癌的生长和发展中发挥作用，并且可能在正常和恶性结肠组织中表达差异。应用逆转录-聚合酶链反应（RT-PCR）、北方印迹分析和免疫组化方法对11例结肠癌进行检测。细胞系和20例具有邻近未受累粘膜的人结肠癌标本中EphB和肝配蛋白-B家族成员的表达。EphB 2、EphB 3和EphB 4 mRNA和ephrin-B2 mRNA在所有细胞系和结肠癌标本中均检测到表达。免疫组化分析显示ephrin-B2在结肠癌组织中的表达高于癌旁正常粘膜，Ephrin-B2和EphB 4主要表达于结肠癌上皮的管腔表面。目前的结果表明，ephrin-Bs在结肠癌和正常粘膜标本中的差异表达，因此可能在结肠癌的进展中发挥作用。需要进一步的研究来确定ephrin-Bs在结肠癌血管生成和生长中的功能作用。(C)2002年美国癌症协会。
BACKGROUND. The erythropoietin-producing hepatoma amplified sequence (Eph) family is the largest subfamily of receptor tyrosine kinases (RTKs). The Ephs (receptors) bind to specific cell-bound ligands, called ephrins. The binding of this ligand-receptor system is dependent on cell-cell interactions. The ephrin-Eph system is important in embryologic development and differentiation of the nervous and vascular systems. In the current study, the authors hypothesized that ephrins may play a role in the growth and development of colon carcinoma and may be expressed differentially in normal and malignant colonic tissues.METHODS. Reverse transcriptase-polymerase chain reaction (RT-PCR), Northern Blot analyses, and immunohistochemistry were used to examine 11 colon carcinoma. cell lines and 20 human colon carcinoma specimens with adjacent uninvolved mucosa for the expression of EphB and ephrin-B family members.RESULTS. EphB2, EphB3, and EphB4 mRNA expression and ephrin-B2 mRNA expression was detected in all the cell lines and colon carcinoma specimens examined. Immunohistochemical analysis showed that ephrin-B2 had higher expression in the colon carcinoma specimens studied than in adjacent normal mucosa.,Ephrin-B2 and EphB4 most frequently were expressed on the luminal surface of colon carcinoma epithelium.CONCLUSIONS. The results of the current suggest that the ephrin-Bs are expressed differentially in colon carcinoma and normal mucosa specimens and thus may play a role in the progression of colon carcinoma. Further studies are necessary to determine the functional role of ephrin-Bs in colon carcinoma angiogenesis and growth. (C) 2002 American Cancer Society.