Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves.
Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves.
复制标题
DOI：
10.1016/j.xpro.2020.100266
复制
发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Gu JG
中科院分区：
文献类型：
--
作者：
Kanda H;Tonomura S;Dai Y;Gu JG
膜片钳记录技术对于研究细胞的离子通道功能是不可或缺的，但应用于朗维尔结是具有挑战性的，朗维尔结是动作电位沿沿着有髓鞘神经传导的关键部位。我们建立了大鼠有髓神经Ranvier结的压力钳膜片钳记录方法。这里描述的一步一步的协议允许研究人员应用这种方法来研究哺乳动物的有髓神经中跳跃传导和信息处理的机制。有关本协议生成和使用的完整信息，请参阅。本文介绍了一种应用于大鼠有髓神经Ranvier结的膜片钳记录技术。它可以用来研究Ranvier结的离子通道及其功能。膜片钳记录技术是研究细胞离子通道功能不可缺少的技术，但应用于Ranvier结是一个具有挑战性的技术。Ranvier结是动作电位沿有髓神经沿着传导的关键部位。我们建立了大鼠有髓神经Ranvier结的压力钳膜片钳记录方法。这里描述的一步一步的协议允许研究人员应用这种方法来研究哺乳动物的有髓神经中跳跃传导和信息处理的机制。
The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals. For complete information on the generation and use of this protocol, please refer to. A pressure-clamped patch-clamp recording technique is developed It is applied to the node of Ranvier of rat myelinated nerves It allows studying ion channels and their functions at the node of Ranvier The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals.
影响因子：
16.2
作者：
Kanda, Hirosato;Ling, Jennifer;Gu, Jianguo G.
通讯作者：
Gu, Jianguo G.