Elevated levels of Th17 cells and Th17-related cytokines are associated with disease activity in patients with inflammatory bowel disease
Elevated levels of Th17 cells and Th17-related cytokines are associated with disease activity in patients with inflammatory bowel disease
复制标题
DOI：
10.1007/s00011-014-0768-7
复制
发表时间：
2014-11-01
影响因子：
6.7
通讯作者：
Zhang, Hongjie
中科院分区：
文献类型：
--
作者：
Jiang, Wenyu;Su, Jiewen;Zhang, Hongjie
产生白细胞介素-17（IL-17）的辅助性T（Th）17细胞被认为是炎症性肠病（IBD）发展的关键细胞的新亚群。为探讨炎症性肠病（IBD）患者肠道Th 17细胞的分布、Th 17相关细胞因子（IL-17、IL-21和IL-22）的表达及其与疾病活动性的关系，我们收集了40例活动期溃疡性结肠炎（UC）患者、20例活动期克罗恩病（CD）患者和20例健康对照者的肠道活检组织。免疫组化法检测结肠组织中Th 17细胞的分布及Th 17相关细胞因子的表达。ELISA法测定血清IL-17、IL-21和IL-22水平。采用Pearson相关分析和斯皮尔曼相关分析分别分析Th 17细胞数、Th 17相关细胞因子表达与疾病活动指数、内镜分级和组织学分级、CRP和PLT水平的相关性。活动期IBD患者IL-21、IL-22水平明显升高（P < 0.05）。Pearson和斯皮尔曼相关分析显示，Th 17细胞数量和Th 17相关细胞因子表达与疾病活动指数、内镜和组织学分级、CRP和PLT水平相关Th 17细胞和Th 17相关细胞因子IL-17、IL-21和IL-22在活动期IBD患者肠粘膜中升高，可能在疾病活动和粘膜损伤中起重要作用。
Interleukin-17(IL-17)-producing T helper(Th)17 cells are considered as a new subset of cells critical to the development of inflammatory bowel disease (IBD). We aimed to investigate the distribution of Th17 cells, the expressions of Th17-related cytokines (IL-17, IL-21 and IL-22) and their association with disease activity in IBD patients.We collected intestinal tissue biopsies from 40 patients with active ulcerative colitis (UC), 20 patients with active Crohn's disease (CD) and 20 healthy controls. The distribution of Th17 cells and expressions of Th17-related cytokines in colonic tissues were evaluated by a standard immunohistochemical procedure. Serum IL-17, IL-21 and IL-22 levels were determined by ELISA. Pearson's and Spearman's correlation analyses were performed to analyze the correlation between the number of Th17 cells, the expressions of Th17-related cytokines and disease activity index, endoscopic and histological grading, and CRP and PLT levels, respectively.Compared with healthy controls, the number of Th17 cells and the expressions of IL-17, IL-21 and IL-22 were significantly increased in active IBD patients (P < 0.05). In addition, Pearson's and Spearman's correlation analyses showed that the number of Th17 cells and the expressions of Th17-related cytokines were correlated with disease activity index, endoscopic and histological grading, CRP and PLT levels (P < 0.05).Th17 cells and Th17-related cytokines (IL-17, IL-21 and IL-22) were increased in the intestinal mucosa in active IBD patients and may play an important role in disease activity and mucosal damage.