Traveling wave model to interpret a wound-healing cell migration assay for human peritoneal mesothelial cells
Traveling wave model to interpret a wound-healing cell migration assay for human peritoneal mesothelial cells
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DOI：
10.1089/107632704323061834
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发表时间：
2004-03-01
影响因子：
--
通讯作者：
Leavesley, DI
中科院分区：
文献类型：
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作者：
Maini, PK;McElwain, DLS;Leavesley, DI
入侵细胞前沿速度的关键决定因素尚不清楚。我们进行了一项“伤口愈合”实验，量化人类腹膜间皮细胞在细胞外基质成分上的迁移。结果根据费希尔方程进行解释，其中包括随机细胞运动（扩散）和增殖建模的术语。该模型预测，在短暂的瞬态之后，入侵的细胞前沿将以行波的形式以恒定的速度移动。这与实验结果一致。使用该模型，获得了细胞增殖速率和扩散系数之间的关系。我们使用该模型推导了控制条件下和 IV 型胶原蛋白存在下的细胞扩散系数，并将其与其他已发表的数据进行了比较。该模型可用于分析癌细胞的侵袭能力以及预测生长因子在组织重建中的功效，包括在皮肤工程中开发单层细胞片或使用培养细胞移植物修复受损的角膜。
The critical determinants of the speed of an invading cell front are not well known. We performed a "wound-healing" experiment that quantifies the migration of human peritoneal mesothelial cells over components of the extracellular matrix. Results were interpreted in terms of Fisher's equation, which includes terms for the modeling of random cell motility (diffusion) and proliferation. The model predicts that, after a short transient, the invading cell front will move as a traveling wave at constant speed. This is consistent with the experimental findings. Using the model, a relationship between the rate of cell proliferation and the diffusion coefficient was obtained. We used the model to deduce the cell diffusion coefficients under control conditions and in the presence of collagen IV and compared these with other published data. The model may be useful in analyzing the invasive capacity of cancer cells as well in predicting the efficacy of growth factors in tissue reconstruction, including the development of monolayer sheets of cells in skin engineering or the repair of injured corneas using grafts of cultured cells.