Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain
Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain
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DOI：
10.1016/s0968-0896(01)00217-6
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发表时间：
2001-09-01
影响因子：
3.5
通讯作者：
Muir, TW
中科院分区：
文献类型：
--
作者：
Camarero, JA;Fushman, D;Muir, TW
在这里，我们描述了在活细胞内进行的第一个多肽化学连接反应的例子。发展了一种基于细胞的天然化学连接方法，并用于从大肠杆菌细胞内的小鼠c-Crk适配器蛋白中产生N端Src同源3(SH3)结构域的环状版本。体内环化反应非常有效，所得到的环状蛋白结构域具有充分的生物活性，并能够采用天然的SH3折叠结构。这项工作代表了在体内产生用于基础生物学研究的小骨架环肽的重要一步。(C)2001爱思唯尔科学有限公司。保留所有权利。
Here we describe the first example of a peptide chemical ligation reaction performed inside a living cell. A cell-based native chemical ligation approach was developed and used to generate a circular version of the N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein inside Escherichia coli cells. The in vivo cyclization reaction was extremely efficient and the resulting circular protein domain was fully biologically active and able to adopt the native SH3 folded structure. This work represents an important step towards the in vivo generation of small backbone cyclic peptides for use in basic biological research. (C) 2001 Elsevier Science Ltd. All rights reserved.