Temperature distribution measurement on microfabricated thermodevice for single biomolecular observation using fluorescent dye
Temperature distribution measurement on microfabricated thermodevice for single biomolecular observation using fluorescent dye
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DOI：
10.1016/j.snb.2005.11.017
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发表时间：
2006-10-12
影响因子：
8.4
通讯作者：
Fujita, Hiroyuki
中科院分区：
文献类型：
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作者：
Arata, Hideyuki F.;Low, Peter;Fujita, Hiroyuki
高空间分辨率的温度分布测量在使用微型热器件的生物检测中具有重要意义。提出了一种利用荧光染料罗丹明B高空间分辨率测量水中温度分布的新方法。由于罗丹明B溶液在其荧光强度中表现出强烈且可逆的温度依赖性变化，因此其可用作温度检测器。根据罗丹明B溶液的荧光强度分布，成功地计算了微型热器件上的温度分布。通过有限元建模验证了该方法的可靠性。测量和模拟的温度分布之间的比较显示出良好的协议。该方法允许直接测量我们的微型thermodevice，其中感兴趣的分子的立场，与3摄氏度的精度和5.3 μ m的空间分辨率的局部温度。由于我们的新方法可以检测温度分布，因此可以沿着光学测量进行精确的温度检测。这为进一步研究F-1-ATPase的温度依赖性提供了一种新的方法。将此方法应用于其它单分子观测，也可实现可靠的温度测量，并取得实质性成果。(c)2005 Elsevier B. V.保留所有权利。
Precise temperature distribution measurements with high spatial resolution are of great importance in bioassay which uses microfabricated thermodevice, such as single biomolecular observation. We propose a new method to measure the temperature distribution in water with high spatial resolution using the fluorescent dye, Rhodamine B. Since Rhodamine B solution exhibits a strong and reversible temperature-dependent variation in its fluorescent intensity, it is useful as a temperature detector. The temperature distribution on a microfabricated thermodevice was successfully calculated from the fluorescent intensity distribution of a Rhodamine B solution. Finite element method modeling was carried out to demonstrate the reliability of the proposed method. A comparison between the measured and simulated temperature distributions revealed an excellent agreement. The method allows for direct measurement of the local temperature on our microfabricated thermodevice, where the molecule of interest stands, with an accuracy of 3 degrees C and a spatial resolution of 5.3 mu m. Precise temperature detection along with optical measurement was possible due to our new method to detect temperature distribution. This is a promising method to reveal the temperature dependent characteristics of F-1-ATPase in future study. Applying this method to other single molecular observations might also realize reliable temperature measurement and may achieve substantial results. (c) 2005 Elsevier B.V. All rights reserved.