Fixed-bed column study of phosphate adsorption using immobilized phosphate-binding protein
Fixed-bed column study of phosphate adsorption using immobilized phosphate-binding protein
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DOI：
10.1016/j.chemosphere.2022.133908
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发表时间：
2022-02-10
期刊：
影响因子：
8.8
通讯作者：
Mayer, Brooke K.
中科院分区：
文献类型：
--
作者：
Hussein, Faten B.;Mayer, Brooke K.
利用高亲和性磷酸盐结合蛋白（PBP）的生物吸附在批量规模的试验中已经证明了有效的磷去除和回收。固定床柱系统的设计和性能的后续优化对于放大和实施是必不可少的。在这里，连续流动固定床柱测试用于研究无机磷酸盐（正磷酸盐，P-i）的吸附，使用固定在树脂上的磷酸盐结合蛋白（PBP-NHS），目标是P-i去除到超低水平，然后回收。突破时间随着流入物Pi浓度的升高、床体积的减小和流入物流速的升高而降低。托马斯和Yoon-Nelson穿透模型充分描述了PBP-NHS树脂性能，相关系数R-2 > 0.95。仅P-i溶液和多离子溶液的穿透曲线的尖锐S形表明使用PBP-NHS树脂高度有利和选择性地分离P-i，超过使用LayneRTTM（一种商业离子交换树脂）实现的分离。PBPNHS柱的P1吸附容量不受竞争阴离子的影响，而LayneRTTM柱的容量下降了20%。在PBP-NHS柱试验中也成功地处理了三级废水流出物，具有典型的S形穿透曲线。以多循环模式操作固定床色谱柱证明了PBP-NHS树脂的可重复使用性，且色谱柱性能无显著下降。本研究的结果有助于努力扩大PBP-NHS吸附系统的设计。
Bio-adsorption using high-affinity phosphate-binding proteins (PBP) has demonstrated effective phosphorus removal and recovery in batch-scale tests. Subsequent optimization of design and performance of fixed-bed column systems is essential for scaling up and implementation. Here, continuous-flow fixed-bed column tests were used to investigate the adsorption of inorganic phosphate (orthophosphate, P-i) using phosphate-binding proteins immobilized on resin (PBP-NHS) targeting P-i removal to ultra-low levels followed by recovery. Time to breakthrough decreased with higher influent P-i concentration, smaller bed volume, and higher influent flow rates. The Thomas and Yoon-Nelson breakthrough models adequately described PBP-NHS resin performance with a correlation coefficient of R-2 > 0.95. The sharp S-shape of the breakthrough curves for both P-i-only solution and multi-ion solution indicated highly favorable and selective separation of P-i using PBP-NHS resin, beyond that achieved using LayneRTTM, a commercial ion exchange resin. The P-i adsorption capacity of the PBPNHS column was unaffected by competing anions, whereas capacity of the LayneRTTM column dropped by 20%. Tertiary wastewater effluent was also successfully treated in PBP-NHS column tests with a typical S-shaped breakthrough curve. Operating the fixed-bed column in multi-cycle mode evidenced the reusability of PBP-NHS resin with no significant decline in column performance. The results of this study contribute to efforts to scale up designs of PBP-NHS adsorption systems.