POSSIBLE SPECIFIC ACTIVATION OF RNA-SYNTHESIS IN PC-12 CELL ISOLATED-NUCLEI BY SMALL ACIDIC PEPTIDES
POSSIBLE SPECIFIC ACTIVATION OF RNA-SYNTHESIS IN PC-12 CELL ISOLATED-NUCLEI BY SMALL ACIDIC PEPTIDES
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DOI：
10.1152/ajpcell.1993.265.5.c1220
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发表时间：
1993-11-01
影响因子：
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通讯作者：
GIANFRANCESCHI, GL
中科院分区：
文献类型：
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作者：
CASTIGLI, E;MANCINELLI, L;GIANFRANCESCHI, GL
三个合成肽,pyro-Glu-Ala-Gly-Glu-Ser-Glu-Asp (Pep), pyro-Glu-Ala-Gly-Glu-Glu-Glu-Glu-Ser-Asn B (Pep),和pyro-Glu-Asp-Asp-Ser-Asp-Glu-Glu-Asn C (Pep),熊序列可能属于一种天然成分密切相关的家庭小酸性染色质肽参与调节基因表达。在PC-12细胞的粗核片段和纯化核中，Pep a和Pep B激活RNA合成，特异性地作用于RNA聚合酶II转录系统。另一方面，Pep C对纯化核的RNA合成有抑制作用，而对粗核部分有激活作用。对照实验表明血清胸腺因子对粗核和纯化核的RNA合成无影响。通过酪蛋白激酶II（在纯化核中比在粗核中更活跃）的肽磷酸化可能的调节进行了讨论。
Three synthetic peptides, pyro-Glu-Ala-Gly-Glu-Ser-Glu-Asp (Pep A), pyro-Glu-Ala-Gly-Glu-Glu-Glu-Glu-Ser-Asn (Pep B), and pyro-Glu-Asp-Asp-Ser-Asp-Glu-Glu-Asn (Pep C), bear sequences possibly belonging to components of a naturally occurring family of strongly related small acidic chromatin peptides involved in regulation of gene expression. In a crude nuclear fraction and in purified nuclei from PC-12 cells, Pep A and Pep B activate RNA synthesis, specifically acting on the RNA polymerase II transcription system. On the other hand, Pep C shows an inhibitory effect on RNA synthesis in purified nuclei but an activation in the crude nuclear fraction. Control experiments show that the serum thymic factor does not affect RNA synthesis in the crude nuclear fraction or in purified nuclei. A possible regulation by peptide phosphorylation via casein kinase II (more active in purified nuclei than in the crude nuclear fraction) is discussed.