Comparative analysis of fecal microbiota in infants with and without eczema.
Comparative analysis of fecal microbiota in infants with and without eczema.
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DOI：
10.1371/journal.pone.0009964
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发表时间：
2010-04-01
期刊：
影响因子：
3.7
通讯作者：
Liu WT
中科院分区：
文献类型：
--
作者：
Hong PY;Lee BW;Aw M;Shek LP;Yap GC;Chua KY;Liu WT
湿疹是一种儿童期慢性疾病，在富裕社会中患病率日益增加。先前的研究假设湿疹的发生与早期生活中地方性微生物群落的微生物特征和组成变化相关，但得出了相互矛盾的结论，这可能是由于没有尽量减少明显的与健康无关的混杂因素（例如年龄、抗生素使用、饮食和分娩方式）。在这项研究中，我们招募了7名剖宫产且完全配方奶喂养的婴儿，并对比检查了这些患有和未患湿疹的婴儿的早期生活地方性微生物群落。通过16S焦磷酸测序，观察到婴儿粪便微生物群落包含变形菌门、厚壁菌门、放线菌门和拟杆菌门这四个主要门类，并且这四个门类中特定种群的存在与否主要受年龄影响。对更大样本量（在1、3和12个月龄时n = 36）的细菌靶点进行定量分析显示，在患有和未患湿疹的剖宫产婴儿中，双歧杆菌和肠杆菌科的丰度不同，并且这些细菌靶点可能是与这些婴儿健康状况相关的潜在生物标志物。我们的总体研究结果表明，在对粪便微生物群落与健康状况进行比较评估和相关性分析之前，尽量减少可能的混杂因素至关重要，并且从1岁以下剖宫产婴儿收集的粪便样本可能是一个很好的队列，用于研究能够区分患有湿疹和未患湿疹婴儿的潜在生物标志物。这些发现将极大地促进未来对某些细菌靶点背后可能的发病机制的理解，并可能导致及时干预，减少早期生活中湿疹的发生以及可能在后期生活中过敏性疾病的发生。
Eczema is a chronic form of childhood disorder that is gaining in prevalence in affluent societies. Previous studies hypothesized that the development of eczema is correlated with changes in microbial profile and composition of early life endemic microbiota, but contradictory conclusions were obtained, possibly due to the lack of minimization of apparent non-health related confounders (e.g., age, antibiotic consumption, diet and mode of delivery). In this study, we recruited seven caesarean-delivered and total formula-fed infants, and comparatively examined the early-life endemic microbiota in these infants with and without eczema. Using 16S pyrosequencing, infants' fecal microbiota were observed to comprise Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the four main phyla, and the presence and absence of specific populations within these four phyla are primarily mediated by ageing. Quantitative analysis of bacterial targets on a larger sample size (n = 36 at 1, 3, and 12 months of age) revealed that the abundances of Bifidobacterium and Enterobacteriaceae were different among caesarean-delivered infants with and without eczema, and the bacterial targets may be potential biomarkers that can correlate to the health status of these infants. Our overall findings suggest that the minimization of possible confounders is essential prior to comparative evaluation and correlation of fecal microbiota to health status, and that stool samples collected from caesarean-delivered infants at less than 1 year of age may represent a good cohort to study for potential biomarkers that can distinguish infants with eczema from those without. These findings would greatly facilitate future efforts in understanding the possible pathogenesis behind certain bacterial targets, and may lead to a timely intervention that reduces the occurrence of early life eczema and possibly allergic disorders in later life.