Creation of a functioning chimeric mammalian kidney.
Creation of a functioning chimeric mammalian kidney.
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DOI：
10.1038/ki.1990.303
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发表时间：
1990-11
影响因子：
19.6
通讯作者：
A. Woolf;A. Woolf;Stephen J. Palmer;Stephen J. Palmer;Michael L. Snow;Michael L. Snow;Leon G. Fine;Leon G. Fine
中科院分区：
文献类型：
--
作者：
A. Woolf;A. Woolf;Stephen J. Palmer;Stephen J. Palmer;Michael L. Snow;Michael L. Snow;Leon G. Fine;Leon G. Fine
创造一个功能齐全的嵌合哺乳动物肾脏。研究了在哺乳动物肾脏中添加新肾元的可能性。将胚胎后肾组织植入出生不足24小时的新生小鼠的肾皮质，并在接下来的2至4周内观察嵌合肾的发育。从纯合子的米色小鼠和转β-珠蛋白基因的小鼠细胞系中获得供体组织，提供了不同的细胞和细胞核标记，用于区分供体和受体肾单位。供体肾单位的分化和生长发生在宿主肾脏，包括血管化的肾小球、成熟的近端肾小管和延伸到肾髓质的肾小管。使用fitc -葡聚糖作为滤过进入近端肾小管的标记物，供体肾细胞可以证实肾小球滤过。将后肾组织移植到成年小鼠肾脏中不会导致肾小球或小管分化。本研究证明了在哺乳动物肾脏中添加功能性肾单位的可行性，这些物种在出生后存在持续的肾形成。
Creation of a functioning chimeric mammalian kidney. The possibility of adding new nephrons to the mammalian kidney was studied. Embryonic metanephric tissue was implanted into the renal cortex of neonatal mice less than 24 hours old, and the development of the chimeric kidney was followed over the following two to four weeks. Donor tissue was obtained from the homozygous beige mouse and a mouse line transgenic for the β-globin gene, which provided distinct cellular and nuclear markers which were used to distinguish donor from recipient nephrons. Differentiation and growth of donor nephrons occurred in the host kidney and included vascularized glomeruli, mature proximal tubules, and tubular extensions into the renal medulla. Glomerular filtration was demonstrable in donor nephrons using FITC-dextran as a marker of filtration into the proximal tubules. Transplantation of metanephric tissue into adult mouse kidneys did not lead to glomerular or tubular differentiation. This study demonstrates the feasibility of adding functioning nephrons to mammalian kidneys in species in which there is ongoing nephrogenesis post-natally.