HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals
HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals
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HLA-D 区 β 链 DNA 核酸内切酶片段在 HLA-DR 相同的健康个体和胰岛素依赖型糖尿病个体之间存在差异
作者：
D. Owerbach;Å. Lernmark;P. Platz;L. Ryder;L. Rask;P. A. Peterson;J. Ludvigsson
人HLA-D组织相容性区编码II类抗原，每种抗原由插入质膜的两条多肽链（α和β）组成。这些分子与免疫应答的调节有关1 -3，但也发现几种人类疾病与某些HLA-DR抗原有关4。胰岛素依赖性（I型）糖尿病（IDDM）的发生与HLA-DR 3和/或4密切相关（参考文献5）。然而，II类抗原显示出与β链相关的显著遗传多态性1，从杂交研究来看，β链似乎由几个基因编码8，9。因此，我们使用β链cDNA探针pDR-β-1（参考文献8，10）来检测在用限制性内切酶消化后，来自健康个体和糖尿病患者的DNA之间的杂交模式是否存在差异。在HLA-DR 4和3/4个体中，IDDM患者显示Pst I 18激酶（kb）片段的频率增加。在对照组中常见的BamHI 3.7kb片段（30-40%）在IDDM患者中很少检测到（0-2%）。这些差异可能与疾病的易感性有关。
The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response1–3 but several human diseases are also found to be associated with certain HLA-DR antigens4. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism1 associated with the β-chains6,7 which seem, from hybridization studies, to be encoded by several genes8,9. We have therefore used the β-chain cDNA probe, pDR-β-1 (refs 8, 10) to test whether there are differences in hybridization pattern between DNA from healthy individuals and diabetic patients, after digestion with restriction endonucleases. Among the HLA-DR 4 and 3/4 individuals, the IDDM patients showed an increased frequency of a PstI 18 kilobase (kb) fragment. A BamHI 3.7 kb fragment, frequent among controls (30–40%), was rarely detected in the IDDM patients (0–2%). These differences may be related to susceptibility to develop the disease.