The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees.
The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees.
复制标题
来自针叶树日本柳杉的 A9 同源物的启动子在转基因树中赋予雄性球果属显性表达。
DOI：
10.1007/s00299-012-1365-2
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发表时间：
2013
影响因子：
6.2
通讯作者：
Toru Taniguchi
中科院分区：
文献类型：
--
作者：
Manabu Kurita;Ken-ichi Konagaya;Atsushi Watanabe;Teiji Kondo;Katsuaki Ishii;Toru Taniguchi
关键信息GUS分析表明，CjMALE 1启动子在柳杉雄球花中被激活。为开发柳杉雄性不育技术，克隆了柳杉雄球花显性启动子。分离出了日本烟草。从雄球花特异性抑制消减杂交（SSH）文库中分离CjMALE 1基因，采用TAIL-PCR方法分离其启动子。为了鉴定CjMALE 1启动子的功能，构建了β-葡萄糖醛酸酶（GUS）融合基因，并将其导入C.根癌农杆菌（Agrobacterium tumefaciens）从CjMALE 1 - 2.5K（2，718 bp片段）：：GUSC的GUS表达。japonica和CjMALE 1 -1 K（1，029 bp片段）：：GUSC。japonica在绒毡层和小孢子母细胞中均有表达。这些启动子片段在C.日本的。我们的分析表明，1，029 bp的启动子具有CjMALE 1雄球花显性表达所需的所有顺式元件。将CjMALE 1 -1 K：：GUS导入拟南芥后，GUS的表达与C.日本的。这些结果表明，theCjMALE 1启动子是受转录调控系统组成的顺式和反式元件，已高度保守的进化过程中。
Key messageGUS analysis inCryptomeria japonicarevealed that theCjMALE1promoter is activated in the male strobilus ofC. japonica.AbstractToward the development of male sterile technology forCryptomeria japonica, a male strobilus-dominant promoter ofC. japonicawas isolated. TheCjMALE1gene was isolated from a male strobilus-specific suppression subtractive hybridization (SSH) library, and the promoter was isolated by the TAIL-PCR method. To characterize theCjMALE1promoter,β-glucuronidase(GUS)-fused genes were constructed and introduced intoC. japonicausingAgrobacterium tumefaciens. GUS expression fromCjMALE1-2.5 K (2,718 bp fragment)::GUSC. japonicaandCjMALE1-1 K (1,029 bp fragment)::GUSC. japonicawas detected in the tapetum and microspore mother cells. These promoter fragments were comparably active in the pre-meiotic stage of the male strobilus ofC. japonica. Our analysis showed that the 1,029 bp promoter had all the cis-elements necessary for male strobilus-dominant expression ofCjMALE1. WhenCjMALE1-1 K::GUSwas introduced intoArabidopsis,GUS expression was detected in the same spatiotemporal pattern as inC. japonica. These results suggest that theCjMALE1promoter is subject to transcriptional regulatory systems consisting of cis- and trans-elements that have been highly conserved during evolution.