A method for the molecular imprinting of hemoglobin on silica surfaces using silanes
A method for the molecular imprinting of hemoglobin on silica surfaces using silanes
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DOI：
10.1016/j.biomaterials.2005.02.007
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Sakaguchi, K
中科院分区：
文献类型：
--
作者：
Shiomi, T;Matsui, M;Sakaguchi, K
本文介绍了一种利用共价键固定血红蛋白（Hb）的分子印迹技术在二氧化硅上形成Hb特异性识别空腔的方法。在通过形成亚胺键共价固定Hb模板之后，将两种有机硅烷（3-氨丙基三甲氧基硅烷：APTMS和三甲氧基丙基硅烷：TMPS）聚合在多孔二氧化硅的表面上，并分析了它们的影响。结果表明，在蛋白质印迹过程中，硅烷用量和相对比例都起着重要的作用。在去除模板Hb后，通过氮气吸附/脱附测定Hb印迹二氧化硅的孔径分布。与其他非模板蛋白相比，使用共价固定化血红蛋白（MIPi）作为模板的血红蛋白印迹二氧化硅证明上级于使用游离血红蛋白（MIPf）的二氧化硅，在模板血红蛋白的置换和选择性再吸附方面。结果表明，MIPi的选择性吸附的能力，不仅是基于等电点（pI）和蛋白质的分子量，但也蛋白质识别腔的基础硅胶印迹的特性。(c)2005爱思唯尔有限公司保留所有权利。
A new molecular imprinting technique using covalently immobilized hemoglobin (Hb) is described for creating Hb-specific recognition cavities on silica. Two kinds of organic silane (3-aminopropyltrimethoxysilane: APTMS, and trimethoxypropylsilane: TMPS) were polymerized on a surface of porous silica after the Hb template was covalently immobilized by forming imine bonds., and their influence was analyzed. The results showed that not only the silane amount but also the relative proportions play an important role in protein imprinting. Pore size distribution on Hb imprinted silica was determined by nitrogen adsorption/ desorption after removing the template Hb. The Hb-imprinted silica using covalently immobilized Hb (MIPi) as a template proved superior to silica using free Hb (MIPf) regarding displacement of template Hb, and selective re-adsorption as compared with other non-template proteins. The results suggested the capacity for selective adsorption of MIPi to be not only based on the isoelectric point (pI) and protein molecular weight, but also the characteristics of protein recognition cavities imprinted on base silica. (c) 2005 Elsevier Ltd. All rights reserved.