In vivo measurement of gene expression, angiogenesis and physiological function in tumors using multiphoton laser scanning microscopy
In vivo measurement of gene expression, angiogenesis and physiological function in tumors using multiphoton laser scanning microscopy
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DOI：
10.1038/89997
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发表时间：
2001-07-01
期刊：
影响因子：
82.9
通讯作者：
Jain, RK
中科院分区：
文献类型：
--
作者：
Brown, EB;Campbell, RB;Jain, RK
活体显微镜与慢性动物窗口模型相结合，为肿瘤病理生理学提供了惊人的见解，包括基因表达，血管生成，细胞粘附和迁移，血管，间质和淋巴运输，代谢微环境和药物递送。然而，迄今为止的发现仅限于肿瘤表面（< 150 m）。在这里，我们表明，多光子激光扫描显微镜可以提供高的三维分辨率的基因表达和功能在更深的区域的肿瘤。这些见解对于开发不仅针对肿瘤表面而且针对内部区域的新型疗法至关重要。
Intravital microscopy coupled with chronic animal window models has provided stunning insight into tumor pathophysiology, including gene expression, angiogenesis, cell adhesion and migration, vascular, interstitial and lymphatic transport, metabolic microenvironment and drug delivery. However, the findings to date have been limited to the tumor surface (< 150 m). Here, we show that the multiphoton laser-scanning microscope can provide high three-dimensional resolution of gene expression and function in deeper regions of tumors. These insights could be critical to the development of novel therapeutics that target not only the tumor surface, but also internal regions.