Towards biocontrol of ophiostomatoid fungi by plant growth-promoting rhizobacteria
Towards biocontrol of ophiostomatoid fungi by plant growth-promoting rhizobacteria
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DOI：
10.1080/09583157.2019.1682517
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发表时间：
2020-01
影响因子：
1.4
通讯作者：
P. Devkota;J. Kloepper;S. Enebak;L. Eckhardt
中科院分区：
文献类型：
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作者：
P. Devkota;J. Kloepper;S. Enebak;L. Eckhardt
摘要蛇口类真菌是引起松树木材蓝变和根部病害的重要病原菌。一些物理，化学和文化的策略，以尽量减少这些真菌的影响是有限的成功。利用植物根际促生菌（PGPR）进行树木病害的生物防治具有很大的吸引力，因为它们可以替代和补充合成化学品而不会对环境产生负面影响。虽然大量的研究表明，PGPR控制农业植物病原体的能力，研究了解PGPR对针叶树的ophiostomatoid真菌病原体的活性仍然缺乏。为了解PGPR在木材蓝变处理中的应用效果，对27株Velezensis芽孢杆菌、Peoriae类芽孢杆菌和B. altitudinis之前显示出对来自六个不同属的植物病原体的抗性，测试了对L. terebrantis和G. Huntii。为了了解PGPR在控制根病中的功效，测试了先前报道对针叶树中的根病原体具有功效的短小芽孢杆菌SE-34和INR 7以及粘质沙雷氏菌（90-166）诱导火炬松对这些真菌的抗性的能力。PGPR菌株对L. terebrantis和G. huntii在体外。在第二个实验中，特定的PGPR处理导致幼苗干物质生物量增加和患病组织减少。结果表明，供试PGPR菌株可能是潜在的生防剂的测试ophiostomatoid真菌。
ABSTRACT Ophiostomatoid fungi, Leptographium terebrantis and Grosmannia huntii, are among the most important agents of wood blue stain and root disease of Pinus species. A number of physical, chemical, and cultural strategies adopted to minimise the impact of these fungi are of limited success. Biological control of tree diseases with plant growth-promoting rhizobacteria (PGPR) is attractive given that they provide an alternative and supplement to synthetic chemicals without negative impact on the environment. While a significant number of studies have shown the ability of PGPR to control pathogens of agricultural plants, studies to understand the activity of PGPR against ophiostomatoid fungal pathogens of conifers is still lacking. To understand the utility of PGPR in treating blue stain in woods, twenty-seven Bacillus velezensis strains and single strains of Paenibacillus peoriae and B. altitudinis that had previously shown antibiosis against plant pathogens from six different genera were tested for in vitro antibiosis against L. terebrantis and G. huntii. To understand the efficacy of PGPR in controlling root-disease, Bacillus pumilus SE-34 and INR7, and Serratia marcescens (90–166), previously reported to have efficacy against a root pathogen in conifer, were tested for their capacity to induce resistance of P. taeda to these fungi. All PGPR strains inhibited the growth of L. terebrantis and G. huntii in vitro. In a second experiment, specific PGPR treatment resulted in increased seedling dry matter biomass and reduction of diseased tissue. The results demonstrate tested PGPR strains may have potential as biocontrol agents to the tested ophiostomatoid fungi.