Magnetic mixed hemimicelles solid-phase extraction of xanthohumol in beer coupled with high-performance liquid chromatography determination
Magnetic mixed hemimicelles solid-phase extraction of xanthohumol in beer coupled with high-performance liquid chromatography determination
复制标题
磁力混合半胶束固相萃取啤酒中黄腐酚高效液相色谱测定
DOI：
10.1002/jssc.201000930
复制
发表时间：
2011-06-01
影响因子：
3.1
通讯作者：
Ren, Nanqi
中科院分区：
文献类型：
--
作者：
Ding, Jie;Zhao, Qi;Ren, Nanqi
本研究合成了十六烷基三甲基溴化铵（CTAB）修饰的二氧化硅包覆磁性纳米颗粒（Fe3O4/SiO2 NPs）。基于磁力混合半胶束固相萃取（MMHSPE）结合高效液相色谱-紫外测定法，成功应用于啤酒中黄腐酚的提取。对影响萃取效率的主要因素包括表面活性剂用量、啤酒pH、萃取时间、解吸条件和最大萃取啤酒体积进行了优化。在优化条件下，用MMHSPE提取120 mL啤酒样品，浓缩倍数为60，黄腐酚的检出限为0.0006 mg/L。该方法成功应用于多种啤酒样品中黄腐酚的测定，黄腐酚含量范围为0.031~0.567 mg/L。在分析加标啤酒样品时获得了令人满意的回收率 (90-103%)。
In this study, silica-coated magnetic nanoparticles (Fe3O4/SiO2 NPs) modified by cetyltrimethylammonium bromide (CTAB) were synthesized. They were successfully applied for extraction of xanthohumol in beer based on magnetic mixed hemimicelles solid-phase extraction (MMHSPE) coupled with high-performance liquid chromatography - ultraviolet determination. The main factors influencing the extraction efficiency including the surfactant amount, the beer pH, the extraction time, the desorption condition and the maximum extraction beer volume were optimized. Under the optimized conditions, a concentration factor of 60 was achieved by extracting 120 mL beer sample using MMHSPE and the detection limit of xanthohumol is 0.0006 mg/L. The proposed method was successfully applied for determination of xanthohumol in various beer samples with the xanthohumol contents in the range of 0.031-0.567 mg/L. The satisfactory recoveries (90-103%) were obtained in analyzing spiked beer samples.