The immunoprotective effect of whole-cell lysed inactivated vaccine with SWCNT as a carrier against Aeromonas hydrophila infection in grass carp
The immunoprotective effect of whole-cell lysed inactivated vaccine with SWCNT as a carrier against Aeromonas hydrophila infection in grass carp
复制标题
SWCNT为载体的全细胞裂解灭活疫苗对草鱼嗜水气单胞菌感染的免疫保护作用
DOI：
10.1016/j.fsi.2019.12.069
复制
发表时间：
2020
影响因子：
4.7
通讯作者：
Fei Ling
中科院分区：
文献类型：
--
作者：
Zhongyu Zhang;Gaoyang Liu;Rui Ma;Xiaozhou Qi;Gaoxue Wang;Bin Zhu;Fei Ling
草鱼气单胞菌是一种革兰氏阴性致病菌，可引起草鱼的大量死亡，造成巨大的经济损失。开发实用的疫苗是控制这种细菌性疾病爆发的最佳途径。本研究通过超声破碎法制备全细胞灭活疫苗，然后将单壁碳纳米管（SWCNTs）与细菌裂解液（BL）连接，制备新型疫苗（SWCNTs-BL）。在用liveA攻击之前，通过浸泡（5、10 mg L-1）或注射（5、10 μg/鱼）用BL和SWCNTs-BL接种总共400条鱼。在免疫后28天（d.p.i.）接种。结果表明，与对照组相比，注射组的抗体效价、酶活性、某些免疫相关基因（尤其是IgMand TNF-α）的表达和RPS在注射后28 d均显著升高。对于浸泡组，免疫学参数与对照组相比增加。SWCNTs-BL的免疫保护作用优于BL。以上结果表明，A.单壁碳纳米管能有效诱导草鱼的特异性免疫应答，BL与功能化单壁碳纳米管的连接能增强浸泡免疫的保护效果。本研究结果为水产养殖业提供了一种生产简便、实用的细菌性病害疫苗。
Aeromonas hydrophila is a strong gram-negative bacterium that can cause a mass death of grass carp, and result in the huge economic loss. Development of practical vaccines is the best way to control the outbreak of this bacterial disease. In this study, a whole-cell inactivated vaccine was obtained via sonication, and then single-walled carbon nanotubes (SWCNTs) was used to link to the bacterial lysate (BL) for a novel vaccine (SWCNTs-BL). A total of 400 fish were vaccinated with BL and SWCNTs-BL via immersion (5, 10 mg L−1) or injection (5, 10 μg/fish) before challenge with liveA. hydrophilaat the 28 days post immunization (d.p.i.). The results showed that the antibody titer, enzymatic activity, expression of some immune-related genes (especiallyIgMandTNF-α) and RPS of fish in the injection groups were significantly increased compared to the control group after 28 d.p.i. For the immersion groups, immunological parameters were increased compared to the control group. Furthermore, the immuno-protective effects of SWCNTs-BL were better than BL. The above results indicated that BL ofA. hydrophilacan effectively induce specific immune response of grass carp, and BL linked with functionalized SWCNTs could enhance the protective effect of immersion immunization. Our results may provide a practical vaccine, with a simple production, to fight against bacterial diseases in aquaculture industry.